↓ Skip to main content

Histones

Overview of attention for book
Cover of 'Histones'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 In Vitro Assembly of Nucleosomes for Binding/Remodeling Assays.
  3. Altmetric Badge
    Chapter 2 An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro.
  4. Altmetric Badge
    Chapter 3 Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Reconstitution and Manipulation of Histone Marks.
  5. Altmetric Badge
    Chapter 4 Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays.
  6. Altmetric Badge
    Chapter 5 Histone Purification from Saccharomyces cerevisiae.
  7. Altmetric Badge
    Chapter 6 Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid-Extracted, HPLC-Purified Histones.
  8. Altmetric Badge
    Chapter 7 SILAC-Based Quantitative Strategies for Accurate Histone Posttranslational Modification Profiling Across Multiple Biological Samples.
  9. Altmetric Badge
    Chapter 8 Characterization of Individual Histone Posttranslational Modifications and Their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies.
  10. Altmetric Badge
    Chapter 9 Production and Purification of Antibodies Against Histone Modifications.
  11. Altmetric Badge
    Chapter 10 Immunofluorescence of Histone Proteins.
  12. Altmetric Badge
    Chapter 11 Acid-Urea Gel Electrophoresis and Western Blotting of Histones.
  13. Altmetric Badge
    Chapter 12 Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast.
  14. Altmetric Badge
    Chapter 13 A Spiking Strategy for ChIP-chip Data Normalization in S. cerevisiae.
  15. Altmetric Badge
    Chapter 14 High-Resolution Genome-Wide Mapping of Nucleosome Positioning and Occupancy Level Using Paired-End Sequencing Technology.
  16. Altmetric Badge
    Chapter 15 Physarum polycephalum for Studying the Function of Histone Modifications In Vivo.
  17. Altmetric Badge
    Chapter 16 A Method for Large-Scale Screening of Random Sequence Libraries to Determine the Function of Unstructured Regions from Essential Proteins.
Attention for Chapter 10: Immunofluorescence of Histone Proteins.
Altmetric Badge

Readers on

mendeley
5 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Immunofluorescence of Histone Proteins.
Chapter number 10
Book title
Histones
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6630-1_10
Pubmed ID
Book ISBNs
978-1-4939-6628-8, 978-1-4939-6630-1
Authors

Hilmar Strickfaden, Michael J. Hendzel

Editors

Benoit Guillemette, Luc R. Gaudreau

Abstract

The identification of a vast array of posttranslational modifications of histone proteins during cell cycle, repair, replication, and transcription has created the challenge of determining structure-function relationships for individual modifications and combinations of modifications. Some of this information can be gathered from indirect immunofluorescence, where the location and cell cycle relationships can be readily identified. Here we present an immunofluorescence protocol that is adapted for the use in histone modifications.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 40%
Professor 1 20%
Researcher 1 20%
Student > Ph. D. Student 1 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 60%
Biochemistry, Genetics and Molecular Biology 2 40%