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Mutant prevention concentration of orbifloxacin: comparison between Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudintermedius of canine origin

Overview of attention for article published in Acta Veterinaria Scandinavica, May 2013
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Title
Mutant prevention concentration of orbifloxacin: comparison between Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudintermedius of canine origin
Published in
Acta Veterinaria Scandinavica, May 2013
DOI 10.1186/1751-0147-55-37
Pubmed ID
Authors

Takae Shimizu, Kazuki Harada, Yasushi Kataoka

Abstract

BACKGROUND: The mutant prevention concentration (MPC) is an important parameter to evaluate the likelihood of growth of fluoroquinolone-resistant mutants for antimicrobial-pathogen combinations. The MPCs of fluoroquinolones for different canine pathogens have not been compared. In this study, we compared for the first time orbifloxacin MPCs between susceptible strains of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudintermedius of canine origin. METHODS: More than 1010 CFU/ml of 10 strains of each bacterial species were inoculated onto Muller-Hinton agar supplemented with different concentrations of orbifloxacin from 1x to 64x minimum inhibitory concentration (MIC) and the MPCs were recorded. MICs of original strains and of mutants arising after exposure to sub-MPC concentrations (one per original strain) were determined in the presence or absence of efflux pump inhibitors (EPIs). The effects of quinolone resistance-determining region (QRDR) mutations were also examined. RESULTS: MPCs were significantly higher for P. aeruginosa (16--128 mug/ml) than for E. coli (0.5--32 mug/ml). MPCs for S. pseudintermedius varied between the low-susceptible (16--128 mug/ml) and the high-susceptible strains (4--16 mug/ml) and were the most broadly distributed among the three species. Regarding resistance mechanisms, only one QRDR mutation in gyrA was found in all of the 10 mutants of E. coli and in 4 of the 10 mutants of P. aeruginosa, whereas mutations in both grlA and gyrA were found in 3 mutants and one mutation in grlA was found in 2 mutants among the 10 mutants of S. pseudintermedius. In the presence of an EPI, the MICs of P. aeruginosa mutants decreased markedly, those of E. coli mutants decreased moderately, and those of S. pseudintermedius mutants were unaffected. CONCLUSIONS: MPCs of orbifloxacin vary between bacterial species of canine pathogens, possibly due to the diversity of the main fluoroquinolone resistance mechanism among these species. Therefore, the type of bacterial species should be taken into consideration when using fluoroquinolone drugs such as orbifloxacin in canines.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Sri Lanka 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 17%
Student > Ph. D. Student 3 13%
Lecturer 2 8%
Student > Bachelor 2 8%
Student > Master 2 8%
Other 3 13%
Unknown 8 33%
Readers by discipline Count As %
Immunology and Microbiology 4 17%
Medicine and Dentistry 4 17%
Agricultural and Biological Sciences 3 13%
Veterinary Science and Veterinary Medicine 1 4%
Biochemistry, Genetics and Molecular Biology 1 4%
Other 2 8%
Unknown 9 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 April 2015.
All research outputs
#16,722,190
of 25,374,917 outputs
Outputs from Acta Veterinaria Scandinavica
#399
of 837 outputs
Outputs of similar age
#124,979
of 204,329 outputs
Outputs of similar age from Acta Veterinaria Scandinavica
#8
of 19 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 837 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 204,329 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.