| Title |
Subcellular location defines GPCR signal transduction
|
|---|---|
| Published in |
Science Advances, April 2023
|
| DOI | 10.1126/sciadv.adf6059 |
| Pubmed ID | |
| Authors |
Arthur Radoux-Mergault, Lucie Oberhauser, Simone Aureli, Francesco Luigi Gervasio, Miriam Stoeber |
| Abstract |
Intracellular G protein-coupled receptors (GPCRs) can be activated by permeant ligands, which contributes to agonist selectivity. Opioid receptors (ORs) provide a notable example, where opioid drugs rapidly activate ORs in the Golgi apparatus. Our knowledge on intracellular GPCR function remains incomplete, and it is unknown whether OR signaling in plasma membrane (PM) and Golgi apparatus differs. Here, we assess the recruitment of signal transducers to mu- and delta-ORs in both compartments. We find that Golgi ORs couple to Gαi/o probes and are phosphorylated but, unlike PM receptors, do not recruit β-arrestin or a specific Gα probe. Molecular dynamics simulations with OR-transducer complexes in bilayers mimicking PM or Golgi composition reveal that the lipid environment promotes the location-selective coupling. We then show that delta-ORs in PM and Golgi have distinct effects on transcription and protein phosphorylation. The study reveals that the subcellular location defines the signaling effects of opioid drugs. |
X Demographics
The data shown below were collected from the profiles of 52 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 6 | 12% |
| Japan | 5 | 10% |
| United States | 4 | 8% |
| Germany | 2 | 4% |
| India | 2 | 4% |
| France | 2 | 4% |
| Canada | 2 | 4% |
| Paraguay | 1 | 2% |
| United Kingdom | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 26 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 29 | 56% |
| Scientists | 21 | 40% |
| Practitioners (doctors, other healthcare professionals) | 2 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 30% |
| Researcher | 9 | 23% |
| Student > Master | 3 | 8% |
| Student > Doctoral Student | 1 | 3% |
| Professor > Associate Professor | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 14 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 25% |
| Agricultural and Biological Sciences | 4 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
| Engineering | 2 | 5% |
| Computer Science | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 18 | 45% |
Attention Score in Context
This research output has an Altmetric Attention Score of 87. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 November 2023.
All research outputs
#502,912
of 25,891,484 outputs
Outputs from Science Advances
#3,581
of 12,632 outputs
Outputs of similar age
#11,232
of 418,883 outputs
Outputs of similar age from Science Advances
#141
of 534 outputs
Altmetric has tracked 25,891,484 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,632 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 119.4. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 418,883 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 534 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.