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Exosomes and Microvesicles

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Cover of 'Exosomes and Microvesicles'

Table of Contents

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    Book Overview
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    Chapter 1 Methods to Analyze EVs.
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    Chapter 2 Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.
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    Chapter 3 Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.
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    Chapter 4 Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.
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    Chapter 5 Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.
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    Chapter 6 Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ and Ion S5™ System.
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    Chapter 7 A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.
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    Chapter 8 Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array).
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    Chapter 9 Purification and Analysis of Exosomes Released by Mature Cortical Neurons Following Synaptic Activation.
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    Chapter 10 A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.
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    Chapter 11 Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.
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    Chapter 12 Isolation of Platelet-Derived Extracellular Vesicles.
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    Chapter 13 Bioinformatics Tools for Extracellular Vesicles Research.
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    Chapter 14 Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.
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    Chapter 15 Interaction of Extracellular Vesicles with Endothelial Cells Under Physiological Flow Conditions.
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    Chapter 16 Flow Cytometric Analysis of Extracellular Vesicles.
Attention for Chapter 3: Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.
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Chapter title
Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.
Chapter number 3
Book title
Exosomes and Microvesicles
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6728-5_3
Pubmed ID
Book ISBNs
978-1-4939-6726-1, 978-1-4939-6728-5
Authors

Kym McNicholas, Michael Z. Michael

Editors

Andrew F Hill

Abstract

Due to their size, small extracellular vesicles such as exosomes have been difficult to identify and to quantify. As the roles that exosomes play in intercellular signalling become clearer, so does their potential utility as both diagnostic biomarkers for disease and as therapeutic vectors. Accurate assessment of exosomes, both their number and their cargo, is important for continued advancement in the field of vesicle research. To that end, several technologies, including nanoparticle tracking analysis, have been developed to define the physical characteristics of vesicle preparations and determine their concentration. This chapter describes a method for identifying the size and concentration of a subpopulation of vesicles in biological samples, using nanoparticle tracking analysis. Characterization of distinct exosomes is enabled by specific marker antibodies, coupled to fluorescent quantum dots.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Israel 1 6%
United States 1 6%
Unknown 15 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 29%
Student > Doctoral Student 3 18%
Researcher 3 18%
Student > Master 3 18%
Professor > Associate Professor 1 6%
Other 1 6%
Unknown 1 6%
Readers by discipline Count As %
Medicine and Dentistry 6 35%
Biochemistry, Genetics and Molecular Biology 6 35%
Agricultural and Biological Sciences 2 12%
Chemistry 1 6%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Other 0 0%
Unknown 1 6%