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Exosomes and Microvesicles

Overview of attention for book
Cover of 'Exosomes and Microvesicles'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Methods to Analyze EVs.
  3. Altmetric Badge
    Chapter 2 Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.
  4. Altmetric Badge
    Chapter 3 Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.
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    Chapter 4 Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.
  6. Altmetric Badge
    Chapter 5 Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.
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    Chapter 6 Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ and Ion S5™ System.
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    Chapter 7 A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.
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    Chapter 8 Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array).
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    Chapter 9 Purification and Analysis of Exosomes Released by Mature Cortical Neurons Following Synaptic Activation.
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    Chapter 10 A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.
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    Chapter 11 Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.
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    Chapter 12 Isolation of Platelet-Derived Extracellular Vesicles.
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    Chapter 13 Bioinformatics Tools for Extracellular Vesicles Research.
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    Chapter 14 Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.
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    Chapter 15 Interaction of Extracellular Vesicles with Endothelial Cells Under Physiological Flow Conditions.
  17. Altmetric Badge
    Chapter 16 Flow Cytometric Analysis of Extracellular Vesicles.
Attention for Chapter 2: Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (69th percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

Mentioned by

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1 news outlet

Citations

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11 Dimensions

Readers on

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48 Mendeley
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Chapter title
Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.
Chapter number 2
Book title
Exosomes and Microvesicles
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6728-5_2
Pubmed ID
Book ISBNs
978-1-4939-6726-1, 978-1-4939-6728-5
Authors

Sybren L. N. Maas, Marike L. D. Broekman, Jeroen de Vrij

Editors

Andrew F Hill

Abstract

Accurate characterization of extracellular vesicles (EVs), including exosomes and microvesicles, is essential to obtain further knowledge on the biological relevance of EVs. Tunable resistive pulse sensing (tRPS) has shown promise as a method for single particle-based quantification and size profiling of EVs. Here, we describe the technical background of tRPS and its applications for EV characterization. Besides the standard protocol, we describe an alternative protocol, in which samples are spiked with polystyrene beads of known size and concentration. This alternative protocol can be used to overcome some of the challenges of direct EV characterization in biological fluids.

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 48 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 29%
Researcher 6 13%
Student > Master 5 10%
Student > Bachelor 5 10%
Student > Doctoral Student 4 8%
Other 9 19%
Unknown 5 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 21%
Biochemistry, Genetics and Molecular Biology 8 17%
Medicine and Dentistry 5 10%
Chemistry 3 6%
Neuroscience 3 6%
Other 7 15%
Unknown 12 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2021.
All research outputs
#5,049,759
of 17,873,421 outputs
Outputs from Methods in molecular biology
#1,396
of 10,239 outputs
Outputs of similar age
#121,116
of 399,388 outputs
Outputs of similar age from Methods in molecular biology
#237
of 1,598 outputs
Altmetric has tracked 17,873,421 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 10,239 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 399,388 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 1,598 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.