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Exosomes and Microvesicles

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Cover of 'Exosomes and Microvesicles'

Table of Contents

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    Book Overview
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    Chapter 1 Methods to Analyze EVs.
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    Chapter 2 Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.
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    Chapter 3 Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.
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    Chapter 4 Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.
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    Chapter 5 Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.
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    Chapter 6 Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ and Ion S5™ System.
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    Chapter 7 A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.
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    Chapter 8 Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array).
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    Chapter 9 Purification and Analysis of Exosomes Released by Mature Cortical Neurons Following Synaptic Activation.
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    Chapter 10 A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.
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    Chapter 11 Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.
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    Chapter 12 Isolation of Platelet-Derived Extracellular Vesicles.
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    Chapter 13 Bioinformatics Tools for Extracellular Vesicles Research.
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    Chapter 14 Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.
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    Chapter 15 Interaction of Extracellular Vesicles with Endothelial Cells Under Physiological Flow Conditions.
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    Chapter 16 Flow Cytometric Analysis of Extracellular Vesicles.
Attention for Chapter 1: Methods to Analyze EVs.
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Chapter title
Methods to Analyze EVs.
Chapter number 1
Book title
Exosomes and Microvesicles
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6728-5_1
Pubmed ID
Book ISBNs
978-1-4939-6726-1, 978-1-4939-6728-5
Authors

Bernd Giebel, Clemens Helmbrecht

Editors

Andrew F Hill

Abstract

Research in the field of extracellular vesicles (EVs) is challenged by the small size of the nano-sized particles. Apart from the use of transmission and scanning electron microscopy, established technical platforms to visualize, quantify, and characterize nano-sized EVs were lacking. Recently, methodologies to characterize nano-sized EVs have been developed. This chapter aims to summarize physical principles of novel and conventional technologies to be used in the EV field and to discuss advantages and limitations.

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 23%
Researcher 5 14%
Student > Bachelor 4 11%
Student > Doctoral Student 4 11%
Student > Master 4 11%
Other 6 17%
Unknown 4 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 16 46%
Medicine and Dentistry 7 20%
Agricultural and Biological Sciences 2 6%
Chemical Engineering 1 3%
Immunology and Microbiology 1 3%
Other 3 9%
Unknown 5 14%