Methods to Analyze EVs.
Exosomes and Microvesicles
Methods in molecular biology, January 2017
Bernd Giebel, Clemens Helmbrecht
Andrew F Hill
Research in the field of extracellular vesicles (EVs) is challenged by the small size of the nano-sized particles. Apart from the use of transmission and scanning electron microscopy, established technical platforms to visualize, quantify, and characterize nano-sized EVs were lacking. Recently, methodologies to characterize nano-sized EVs have been developed. This chapter aims to summarize physical principles of novel and conventional technologies to be used in the EV field and to discuss advantages and limitations.
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