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Exosomes and Microvesicles

Overview of attention for book
Cover of 'Exosomes and Microvesicles'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Methods to Analyze EVs.
  3. Altmetric Badge
    Chapter 2 Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.
  4. Altmetric Badge
    Chapter 3 Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.
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    Chapter 4 Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.
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    Chapter 5 Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.
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    Chapter 6 Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ and Ion S5™ System.
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    Chapter 7 A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.
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    Chapter 8 Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array).
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    Chapter 9 Purification and Analysis of Exosomes Released by Mature Cortical Neurons Following Synaptic Activation.
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    Chapter 10 A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.
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    Chapter 11 Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.
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    Chapter 12 Isolation of Platelet-Derived Extracellular Vesicles.
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    Chapter 13 Bioinformatics Tools for Extracellular Vesicles Research.
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    Chapter 14 Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.
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    Chapter 15 Interaction of Extracellular Vesicles with Endothelial Cells Under Physiological Flow Conditions.
  17. Altmetric Badge
    Chapter 16 Flow Cytometric Analysis of Extracellular Vesicles.
Attention for Chapter 4: Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

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1 blog

Citations

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Readers on

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40 Mendeley
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Chapter title
Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.
Chapter number 4
Book title
Exosomes and Microvesicles
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6728-5_4
Pubmed ID
Book ISBNs
978-1-4939-6726-1, 978-1-4939-6728-5
Authors

Romain Linares, Sisareuth Tan, Céline Gounou, Alain R. Brisson, Linares, Romain, Tan, Sisareuth, Gounou, Céline, Brisson, Alain R.

Editors

Andrew F Hill

Abstract

Extracellular vesicles (EVs) are cell-derived vesicles that are present in blood and other body fluids. EVs raise major interest for their diverse physiopathological roles and their potential biomedical applications. However, the characterization and quantification of EVs constitute major challenges, mainly due to their small size and the lack of methods adapted for their study. Electron microscopy has made significant contributions to the EV field since their initial discovery. Here, we describe the use of two transmission electron microscopy (TEM) techniques for imaging and quantifying EVs. Cryo-TEM combined with receptor-specific gold labeling is applied to reveal the morphology, size, and phenotype of EVs, while their enumeration is achieved after high-speed sedimentation on EM grids.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 28%
Researcher 8 20%
Student > Master 6 15%
Student > Bachelor 5 13%
Professor 3 8%
Other 3 8%
Unknown 4 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 23%
Biochemistry, Genetics and Molecular Biology 8 20%
Immunology and Microbiology 4 10%
Medicine and Dentistry 3 8%
Chemistry 2 5%
Other 6 15%
Unknown 8 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 April 2019.
All research outputs
#3,529,536
of 14,714,661 outputs
Outputs from Methods in molecular biology
#927
of 8,776 outputs
Outputs of similar age
#99,332
of 380,369 outputs
Outputs of similar age from Methods in molecular biology
#179
of 1,569 outputs
Altmetric has tracked 14,714,661 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,776 research outputs from this source. They receive a mean Attention Score of 2.5. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 380,369 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 1,569 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.