| Chapter title |
Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.
|
|---|---|
| Chapter number | 14 |
| Book title |
Exosomes and Microvesicles
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6728-5_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6726-1, 978-1-4939-6728-5
|
| Authors |
Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir E. L. Andaloussi, Matthew J. A. Wood |
| Editors |
Andrew F Hill |
| Abstract |
RNA interference (RNAi) has tremendous potential for specific silencing of disease-causing genes. Its clinical usage however critically depends on the development of carrier systems that can transport the RNAi-mediating small interfering RNA (siRNA) molecules to the cytosol of target cells. Recent reports have suggested that extracellular vesicles (EVs) form a natural transport system through which biomolecules, including RNA, is exchanged between cells. Therefore, EVs are increasingly being considered as potential therapeutic siRNA delivery systems.In this chapter we describe a method for preparing siRNA-loaded EVs, including a robust, scalable method to isolate them from cell culture supernatants. |
Mendeley readers
Geographical breakdown
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|---|---|---|
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| Unknown | 20 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 29% |
| Student > Master | 5 | 24% |
| Student > Doctoral Student | 4 | 19% |
| Researcher | 2 | 10% |
| Unspecified | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 3 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Medicine and Dentistry | 3 | 14% |
| Neuroscience | 2 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 3 | 14% |