Chapter title |
Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.
|
---|---|
Chapter number | 14 |
Book title |
Exosomes and Microvesicles
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6728-5_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6726-1, 978-1-4939-6728-5
|
Authors |
Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir E. L. Andaloussi, Matthew J. A. Wood |
Editors |
Andrew F Hill |
Abstract |
RNA interference (RNAi) has tremendous potential for specific silencing of disease-causing genes. Its clinical usage however critically depends on the development of carrier systems that can transport the RNAi-mediating small interfering RNA (siRNA) molecules to the cytosol of target cells. Recent reports have suggested that extracellular vesicles (EVs) form a natural transport system through which biomolecules, including RNA, is exchanged between cells. Therefore, EVs are increasingly being considered as potential therapeutic siRNA delivery systems.In this chapter we describe a method for preparing siRNA-loaded EVs, including a robust, scalable method to isolate them from cell culture supernatants. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Denmark | 1 | 3% |
Unknown | 32 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 21% |
Student > Ph. D. Student | 6 | 18% |
Student > Master | 5 | 15% |
Student > Doctoral Student | 3 | 9% |
Student > Bachelor | 2 | 6% |
Other | 0 | 0% |
Unknown | 10 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 27% |
Medicine and Dentistry | 5 | 15% |
Agricultural and Biological Sciences | 4 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Neuroscience | 2 | 6% |
Other | 0 | 0% |
Unknown | 11 | 33% |