Chapter title |
Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.
|
---|---|
Chapter number | 14 |
Book title |
Exosomes and Microvesicles
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6728-5_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6726-1, 978-1-4939-6728-5
|
Authors |
Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir E. L. Andaloussi, Matthew J. A. Wood |
Editors |
Andrew F Hill |
Abstract |
RNA interference (RNAi) has tremendous potential for specific silencing of disease-causing genes. Its clinical usage however critically depends on the development of carrier systems that can transport the RNAi-mediating small interfering RNA (siRNA) molecules to the cytosol of target cells. Recent reports have suggested that extracellular vesicles (EVs) form a natural transport system through which biomolecules, including RNA, is exchanged between cells. Therefore, EVs are increasingly being considered as potential therapeutic siRNA delivery systems.In this chapter we describe a method for preparing siRNA-loaded EVs, including a robust, scalable method to isolate them from cell culture supernatants. |
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Geographical breakdown
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Demographic breakdown
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Student > Master | 5 | 22% |
Student > Doctoral Student | 4 | 17% |
Researcher | 4 | 17% |
Unknown | 4 | 17% |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 0 | 0% |
Unknown | 4 | 17% |