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Modern Proteomics – Sample Preparation, Analysis and Practical Applications

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Cover of 'Modern Proteomics – Sample Preparation, Analysis and Practical Applications'

Table of Contents

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    Book Overview
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    Chapter 1 Proteomes, Their Compositions and Their Sources
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    Chapter 2 Protein Fractionation and Enrichment Prior to Proteomics Sample Preparation
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    Chapter 3 Sample Preparation for Mass Spectrometry-Based Proteomics; from Proteomes to Peptides
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    Chapter 4 Plant Structure and Specificity – Challenges and Sample Preparation Considerations for Proteomics
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    Chapter 5 Improving Proteome Coverage by Reducing Sample Complexity via Chromatography
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    Chapter 6 Database Search Engines: Paradigms, Challenges and Solutions
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    Chapter 7 Mass Analyzers and Mass Spectrometers
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    Chapter 8 Top-Down Mass Spectrometry: Proteomics to Proteoforms
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    Chapter 9 Platforms and Pipelines for Proteomics Data Analysis and Management
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    Chapter 10 Tandem Mass Spectrum Sequencing: An Alternative to Database Search Engines in Shotgun Proteomics
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    Chapter 11 Visualization, Inspection and Interpretation of Shotgun Proteomics Identification Results
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    Chapter 12 Protein Inference
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    Chapter 13 Modification Site Localization in Peptides
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    Chapter 14 Useful Web Resources
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    Chapter 15 Mass Spectrometry-Based Protein Quantification
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    Chapter 16 Bioinformatics Tools for Proteomics Data Interpretation
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    Chapter 17 Identification, Quantification, and Site Localization of Protein Posttranslational Modifications via Mass Spectrometry-Based Proteomics
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    Chapter 18 Protein-Protein Interaction Detection Via Mass Spectrometry-Based Proteomics
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    Chapter 19 Protein Structural Analysis via Mass Spectrometry-Based Proteomics
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    Chapter 20 Introduction to Clinical Proteomics
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    Chapter 21 Discovery of Candidate Biomarkers
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    Chapter 22 Statistical Approaches to Candidate Biomarker Panel Selection
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    Chapter 23 Qualification and Verification of Protein Biomarker Candidates
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    Chapter 24 Protocol for Standardizing High-to-Moderate Abundance Protein Biomarker Assessments Through an MRM-with-Standard-Peptides Quantitative Approach
Attention for Chapter 6: Database Search Engines: Paradigms, Challenges and Solutions
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Chapter title
Database Search Engines: Paradigms, Challenges and Solutions
Chapter number 6
Book title
Modern Proteomics – Sample Preparation, Analysis and Practical Applications
Published in
Advances in experimental medicine and biology, December 2016
DOI 10.1007/978-3-319-41448-5_6
Pubmed ID
Book ISBNs
978-3-31-941446-1, 978-3-31-941448-5
Authors

Kenneth Verheggen, Lennart Martens, Frode S. Berven, Harald Barsnes, Marc Vaudel

Editors

Hamid Mirzaei, Martin Carrasco

Abstract

The first step in identifying proteins from mass spectrometry based shotgun proteomics data is to infer peptides from tandem mass spectra, a task generally achieved using database search engines. In this chapter, the basic principles of database search engines are introduced with a focus on open source software, and the use of database search engines is demonstrated using the freely available SearchGUI interface. This chapter also discusses how to tackle general issues related to sequence database searching and shows how to minimize their impact.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 51 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 22%
Student > Master 8 16%
Researcher 6 12%
Other 3 6%
Student > Doctoral Student 2 4%
Other 6 12%
Unknown 15 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 27%
Agricultural and Biological Sciences 4 8%
Engineering 2 4%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Computer Science 2 4%
Other 7 14%
Unknown 20 39%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 October 2017.
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#18,493,111
of 22,914,829 outputs
Outputs from Advances in experimental medicine and biology
#3,316
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#309,685
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Outputs of similar age from Advances in experimental medicine and biology
#338
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