| Chapter title |
Top-Down Mass Spectrometry: Proteomics to Proteoforms
|
|---|---|
| Chapter number | 8 |
| Book title |
Modern Proteomics – Sample Preparation, Analysis and Practical Applications
|
| Published in |
Advances in experimental medicine and biology, December 2016
|
| DOI | 10.1007/978-3-319-41448-5_8 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-941446-1, 978-3-31-941448-5
|
| Authors |
Steven M. Patrie |
| Editors |
Hamid Mirzaei, Martin Carrasco |
| Abstract |
This chapter highlights many of the fundamental concepts and technologies in the field of top-down mass spectrometry (TDMS), and provides numerous examples of contributions that TD is making in biology, biophysics, and clinical investigations. TD workflows include variegated steps that may include non-specific or targeted preparative strategies, orthogonal liquid chromatography techniques, analyte ionization, mass analysis, tandem mass spectrometry (MS/MS) and informatics procedures. This diversity of experimental designs has evolved to manage the large dynamic range of protein expression and diverse physiochemical properties of proteins in proteome investigations, tackle proteoform microheterogeneity, as well as determine structure and composition of gas-phase proteins and protein assemblies. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 23% |
| Student > Master | 9 | 23% |
| Student > Bachelor | 3 | 8% |
| Other | 3 | 8% |
| Student > Doctoral Student | 2 | 5% |
| Other | 7 | 18% |
| Unknown | 7 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 30% |
| Medicine and Dentistry | 6 | 15% |
| Agricultural and Biological Sciences | 4 | 10% |
| Chemistry | 4 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 5 | 13% |
| Unknown | 8 | 20% |