| Chapter title |
Chromosome Preparation for Chronic Lymphoid Malignancies.
|
|---|---|
| Chapter number | 4 |
| Book title |
Cancer Cytogenetics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6703-2_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6701-8, 978-1-4939-6703-2
|
| Authors |
Dorota Koczkodaj, Agata A. Filip |
| Editors |
Thomas S.K. Wan |
| Abstract |
Conventional cytogenetics is invariably one of the most important methods used in diagnostics of chronic lymphoproliferations. It complements fluorescence in situ hybridization (FISH) and molecular analysis. Presence of particular chromosomal alterations in chronic lymphocytic leukemia enables patients' stratification into appropriate cytogenetic risk groups and influences treatment decisions. In other non-Hodgkin lymphomas cytogenetic analyses are employed also in minimal residual disease assessment.As lymphocytes in chronic lymphoid malignancies are characterized by low proliferation rate in vitro, it is critical to induce their division in the culture properly. Here, we describe methods of lymphocyte isolation from patient's samples, conditions of cell culture, and the most commonly used mitogens for B- and T-lymphocytes in hemato-oncologic analyses. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 40% |
| Librarian | 1 | 20% |
| Researcher | 1 | 20% |
| Lecturer > Senior Lecturer | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 80% |
| Medicine and Dentistry | 1 | 20% |