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Cancer Cytogenetics

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Cover of 'Cancer Cytogenetics'

Table of Contents

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    Book Overview
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    Chapter 1 Cancer Cytogenetics: An Introduction.
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    Chapter 2 Chromosome Preparation for Myeloid Malignancies.
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    Chapter 3 Chromosome Preparation for Acute Lymphoblastic Leukemia.
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    Chapter 4 Chromosome Preparation for Chronic Lymphoid Malignancies.
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    Chapter 5 Cytogenetic Harvesting of Cancer Cells and Cell Lines.
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    Chapter 6 Chromosome Bandings.
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    Chapter 7 Chromosome Recognition.
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    Chapter 8 Applications of Fluorescence In Situ Hybridization Technology in Malignancies.
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    Chapter 9 Fluorescence In Situ Hybridization Probe Preparation.
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    Chapter 10 Fluorescence In Situ Hybridization Probe Validation for Clinical Use.
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    Chapter 11 Fluorescence In Situ Hybridization on Tissue Sections.
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    Chapter 12 Cytoplasmic Immunoglobulin Light Chain Revelation and Interphase Fluorescence In Situ Hybridization in Myeloma.
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    Chapter 13 Quantitative Fluorescence In Situ Hybridization (QFISH).
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    Chapter 14 High Resolution Fiber-Fluorescence In Situ Hybridization.
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    Chapter 15 Array-Based Comparative Genomic Hybridization (aCGH).
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    Chapter 16 Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND).
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    Chapter 17 Cytogenetics for Biological Dosimetry.
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    Chapter 18 Recurrent Cytogenetic Abnormalities in Myelodysplastic Syndromes.
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    Chapter 19 Recurrent Cytogenetic Abnormalities in Acute Myeloid Leukemia.
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    Chapter 20 Recurrent Cytogenetic Abnormalities in Myeloproliferative Neoplasms and Chronic Myeloid Leukemia.
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    Chapter 21 Recurrent Cytogenetic Abnormalities in Acute Lymphoblastic Leukemia.
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    Chapter 22 Recurrent Cytogenetic Abnormalities in Non-Hodgkin's Lymphoma and Chronic Lymphocytic Leukemia.
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    Chapter 23 Recurrent Cytogenetic Abnormalities in Multiple Myeloma.
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    Chapter 24 Cytogenetic Nomenclature and Reporting.
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    Chapter 25 Cytogenetic Resources and Information.
Attention for Chapter 13: Quantitative Fluorescence In Situ Hybridization (QFISH).
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Chapter title
Quantitative Fluorescence In Situ Hybridization (QFISH).
Chapter number 13
Book title
Cancer Cytogenetics
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6703-2_13
Pubmed ID
Book ISBNs
978-1-4939-6701-8, 978-1-4939-6703-2
Authors

Ivan Y. Iourov

Editors

Thomas S.K. Wan

Abstract

Fluorescence in situ hybridization (FISH) has a wide spectrum of applications in current molecular cytogenetic and cancer research. This is a unique technique that can be used for chromosomal DNA analysis in all cell types, at all stages of the cell cycle, and at molecular resolution. Recent developments in microscopy and imaging systems have allowed quantification of digital FISH images (quantitative FISH or QFISH) and have provided a new way for molecular cytogenetic analysis at single-cell level. QFISH can be applied for studying chromosome imbalances in interphase nuclei or metaphase spreads, measuring relative DNA content at chromosomal loci and identifying parental origin of homologous chromosomes. Here, a QFISH protocol suitable for the majority of DNA probes using the popular US National Institute of Health developed ImageJ software is described.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 2 3%
Iran, Islamic Republic of 1 1%
United States 1 1%
Canada 1 1%
Unknown 74 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 20 25%
Student > Ph. D. Student 19 24%
Student > Doctoral Student 8 10%
Student > Bachelor 5 6%
Other 4 5%
Other 12 15%
Unknown 11 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 38%
Biochemistry, Genetics and Molecular Biology 13 16%
Medicine and Dentistry 5 6%
Immunology and Microbiology 4 5%
Chemistry 4 5%
Other 9 11%
Unknown 14 18%