Chapter title |
Immunohistochemical Analysis of mTOR Activity in Tissues.
|
---|---|
Chapter number | 12 |
Book title |
mTOR
|
Published in |
Methods in molecular biology, September 2011
|
DOI | 10.1007/978-1-61779-430-8_12 |
Pubmed ID | |
Book ISBNs |
978-1-61779-429-2, 978-1-61779-430-8
|
Authors |
Jinhee Kim, Nancy Otto, Claudio J. Conti, Irma B. Gimenz-Conti, Cheryl L. Walker |
Editors |
Thomas Weichhart |
Abstract |
mTOR is a key regulator of cell growth and size, and its activity is often dysregulated in a wide variety of diseases. The mTOR signaling pathway is also a therapeutic target for many diseases, including cancer. Immunohistochemistry is a powerful method to assess mTOR activity in clinical/histological samples, however, care should be taken in choosing the targets for determining mTOR activity due to the complexity of its regulation. This chapter describes the most up-to-date methods for visualizing mTOR activity by immunohistochemistry using commercially available antibodies, including considerations for validating new antibodies for assessing mTOR signaling. |
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