Title |
Multiplex target capture with double-stranded DNA probes
|
---|---|
Published in |
Genome Medicine, May 2013
|
DOI | 10.1186/gm454 |
Pubmed ID | |
Authors |
Peidong Shen, Wenyi Wang, Aung-Kyaw Chi, Yu Fan, Ronald W Davis, Curt Scharfe |
Abstract |
Target enrichment technologies utilize single-stranded oligonucleotide probes to capture candidate genomic regions from a DNA sample before sequencing. We describe target capture using double-stranded probes, which consist of single-stranded, complementary long padlock probes (cLPPs), each selectively capturing one strand of a genomic target through circularization. Using two probes per target increases sensitivity for variant detection and cLPPs are easily produced by PCR at low cost. Additionally, we introduce an approach for generating capture libraries with uniformly randomized template orientations. This facilitates bidirectional sequencing of both the sense and antisense template strands during one paired-end read, which maximizes target coverage. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
United Kingdom | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 2% |
France | 1 | 1% |
Switzerland | 1 | 1% |
Canada | 1 | 1% |
United Kingdom | 1 | 1% |
Unknown | 77 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 26 | 31% |
Researcher | 26 | 31% |
Student > Master | 6 | 7% |
Other | 5 | 6% |
Professor > Associate Professor | 3 | 4% |
Other | 7 | 8% |
Unknown | 10 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 44 | 53% |
Biochemistry, Genetics and Molecular Biology | 19 | 23% |
Chemistry | 3 | 4% |
Medicine and Dentistry | 2 | 2% |
Veterinary Science and Veterinary Medicine | 1 | 1% |
Other | 3 | 4% |
Unknown | 11 | 13% |