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MALDI-TOF MS as an innovative tool for detection of Plasmodium parasites in Anopheles mosquitoes

Overview of attention for article published in Malaria Journal, January 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (76th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

Mentioned by

twitter
6 tweeters
wikipedia
1 Wikipedia page

Citations

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23 Dimensions

Readers on

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45 Mendeley
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Title
MALDI-TOF MS as an innovative tool for detection of Plasmodium parasites in Anopheles mosquitoes
Published in
Malaria Journal, January 2017
DOI 10.1186/s12936-016-1657-z
Pubmed ID
Authors

Maureen Laroche, Lionel Almeras, Emilie Pecchi, Yassina Bechah, Didier Raoult, Angèle Viola, Philippe Parola

Abstract

Malaria is still a major public health issue worldwide, and one of the best approaches to fight the disease remains vector control. The current methods for mosquito identification include morphological methods that are generally time-consuming and require expertise, and molecular methods that require laboratory facilities with relatively expensive running costs. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technology, routinely used for bacterial identification, has recently emerged in the field of entomology. The aim of the present study was to assess whether MALDI-TOF MS could successfully distinguish Anopheles stephensi mosquitoes according to their Plasmodium infection status. C57BL/6 mice experimentally infected with Plasmodium berghei were exposed to An. stephensi bites. For the determination of An. stephensi infection status, mosquito cephalothoraxes were dissected and submitted to mass spectrometry analyses and DNA amplification for molecular analysis. Spectra were grouped according to mosquitoes' infection status and spectra quality was validated based on intensity and reproducibility within each group. The in-lab MALDI-TOF MS arthropod reference spectra database, upgraded with representative spectra from both groups (infected/non-infected), was subsequently queried blindly with cephalothorax spectra from specimens of both groups. The MALDI TOF MS profiles generated from protein extracts prepared from the cephalothorax of An. stephensi allowed distinction between infected and uninfected mosquitoes. Correct classification was obtained in blind test analysis for (79/80) 98.75% of all mosquitoes tested. Only one of 80 specimens, an infected mosquito, was misclassified in the blind test analysis. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry appears to be a promising, rapid and reliable tool for the epidemiological surveillance of Anopheles vectors, including their identification and their infection status.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
Madagascar 1 2%
Ghana 1 2%
Unknown 42 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 20%
Researcher 9 20%
Student > Master 7 16%
Student > Postgraduate 5 11%
Student > Doctoral Student 4 9%
Other 8 18%
Unknown 3 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 40%
Biochemistry, Genetics and Molecular Biology 6 13%
Immunology and Microbiology 6 13%
Medicine and Dentistry 5 11%
Computer Science 1 2%
Other 2 4%
Unknown 7 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 April 2019.
All research outputs
#2,537,182
of 13,998,077 outputs
Outputs from Malaria Journal
#721
of 4,026 outputs
Outputs of similar age
#88,367
of 376,804 outputs
Outputs of similar age from Malaria Journal
#91
of 560 outputs
Altmetric has tracked 13,998,077 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,026 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 376,804 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 560 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.