Chapter title |
Visualization of the T Cell Response in Contact Hypersensitivity
|
---|---|
Chapter number | 4 |
Book title |
Inflammation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6786-5_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6784-1, 978-1-4939-6786-5
|
Authors |
Gyohei Egawa M.D., Ph.D., Kenji Kabashima, Gyohei Egawa |
Editors |
Björn E. Clausen, Jon D. Laman |
Abstract |
Contact hypersensitivity (CHS) is the most basic murine model for type IV hypersensitivity. This dermatitis model is mediated by hapten-sensitized, skin-infiltrating T cells. Recent intravital imaging studies have demonstrated the dynamic migratory property of skin-infiltrating T cells and the orchestrated interaction of T cells with dermal dendritic cells. Multiphoton microscopy enables the direct, three-dimensional, and minimally invasive imaging of in vivo skin samples with high spatiotemporal resolution. Here, we describe a basic method for the intravital imaging of skin-infiltrating T cells in the DNFB-induced CHS response. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 2 | 29% |
Student > Bachelor | 1 | 14% |
Professor > Associate Professor | 1 | 14% |
Unknown | 3 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
Nursing and Health Professions | 1 | 14% |
Immunology and Microbiology | 1 | 14% |
Medicine and Dentistry | 1 | 14% |
Unknown | 3 | 43% |