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Inflammation

Overview of attention for book
Cover of 'Inflammation'

Table of Contents

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    Book Overview
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    Chapter 1 Reproducibility Issues: Avoiding Pitfalls in Animal Inflammation Models
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    Chapter 2 Hapten-Specific T Cell-Mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate
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    Chapter 3 Monitoring Skin Dendritic Cells in Steady State and Inflammation by Immunofluorescence Microscopy and Flow Cytometry
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    Chapter 4 Visualization of the T Cell Response in Contact Hypersensitivity
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    Chapter 5 Ultraviolet Radiation-Induced Immunosuppression: Induction of Regulatory T Cells
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    Chapter 6 Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Model
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    Chapter 7 Xenotransplantation Model of Psoriasis
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    Chapter 8 A Mouse Model for Atopic Dermatitis Using Topical Application of Vitamin D3 or of Its Analog MC903
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    Chapter 9 Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins
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    Chapter 10 Murine Models of Allergic Asthma
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    Chapter 11 Subcutaneous and Sublingual Immunotherapy in a Mouse Model of Allergic Asthma
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    Chapter 12 Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse
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    Chapter 13 Induction and Analysis of Bronchus-Associated Lymphoid Tissue
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    Chapter 14 Messenger RNA Sequencing of Rare Cell Populations in the Lung and Lung-Draining Lymph Nodes
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    Chapter 15 Isolation and Identification of Intestinal Myeloid Cells
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    Chapter 16 Monitoring and Modulation of Inducible Foxp3+ Regulatory T-Cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon
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    Chapter 17 Isolation and Flow Cytometry Analysis of Innate Lymphoid Cells from the Intestinal Lamina Propria
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    Chapter 18 Analysis of Leukocytes in Oral Mucosal Tissues
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    Chapter 19 Optimized Mouse Models for Liver Fibrosis
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    Chapter 20 Monitoring of Chemically Induced Colitis
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    Chapter 21 Oxazolone-Induced Intestinal Inflammation in Adult Zebrafish
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    Chapter 22 High Dimensional Cytometry of Central Nervous System Leukocytes During Neuroinflammation
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    Chapter 23 Isolation of Microglia and Immune Infiltrates from Mouse and Primate Central Nervous System
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    Chapter 24 Investigating the Lymphatic Drainage of the Brain: Essential Skills and Tools
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    Chapter 25 Quantitative Assessment of Cerebral Basement Membranes Using Electron Microscopy
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    Chapter 26 Microglial Activation by Genetically Targeted Conditional Neuronal Ablation in the Zebrafish
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    Chapter 27 Experimental Arthritis Mouse Models Driven by Adaptive and/or Innate Inflammation
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    Chapter 28 Pain Relief in Nonhuman Primate Models of Arthritis
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    Chapter 29 Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis
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    Chapter 30 Assessment of Vascular Dysfunction and Inflammation Induced by Angiotensin II in Mice
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    Chapter 31 Erratum to: Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis
Attention for Chapter 15: Isolation and Identification of Intestinal Myeloid Cells
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Citations

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Chapter title
Isolation and Identification of Intestinal Myeloid Cells
Chapter number 15
Book title
Inflammation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6786-5_15
Pubmed ID
28063047
Book ISBNs
978-1-4939-6784-1, 978-1-4939-6786-5
Authors

Charlotte L. Scott, Calum C. Bain, Allan McI Mowat, Scott, Charlotte L., Bain, Calum C., Mowat, Allan McI

Editors

Björn E. Clausen, Jon D. Laman

Abstract

The identification of conventional dendritic cells (cDCs) and macrophages (mϕ) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from each other and from other intestinal myeloid cells. Here we detail the protocols we have developed to isolate live leukocytes from steady state mouse small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs, monocytes and macrophages in such preparations.

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Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 21%
Other 3 16%
Student > Doctoral Student 2 11%
Student > Master 2 11%
Professor 1 5%
Other 2 11%
Unknown 5 26%
Readers by discipline Count As %
Immunology and Microbiology 5 26%
Biochemistry, Genetics and Molecular Biology 2 11%
Medicine and Dentistry 2 11%
Agricultural and Biological Sciences 2 11%
Arts and Humanities 1 5%
Other 2 11%
Unknown 5 26%

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