| Chapter title |
Isolation of Microglia and Immune Infiltrates from Mouse and Primate Central Nervous System
|
|---|---|
| Chapter number | 23 |
| Book title |
Inflammation
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6786-5_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6784-1, 978-1-4939-6786-5
|
| Authors |
Thais F. Galatro, Ilia D. Vainchtein, Nieske Brouwer, Erik W. G. M. Boddeke, Bart J. L. Eggen |
| Editors |
Björn E. Clausen, Jon D. Laman |
| Abstract |
Microglia are the innate immune cells of the central nervous system (CNS) and play an important role in the maintenance of tissue homeostasis, providing neural support and neuroprotection. Microglia constantly survey their environment and quickly respond to homeostatic perturbations. Microglia are increasingly implicated in neuropathological and neurodegenerative conditions, such as Alzheimer's disease, Parkinson's disease, and glioma progression. Here, we describe a detailed isolation protocol for microglia and immune infiltrates, optimized for large amounts of post mortem tissue from human and rhesus macaque, as well as smaller tissue amounts from mouse brain and spinal cord, that yield a highly purified microglia population (up to 98 % purity). This acute isolation protocol is based on mechanical dissociation and a two-step density gradient purification, followed by fluorescence-activated cell sorting (FACS) to obtain pure microglia and immune infiltrate populations. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 77 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 31% |
| Student > Master | 11 | 14% |
| Student > Bachelor | 8 | 10% |
| Researcher | 8 | 10% |
| Student > Doctoral Student | 6 | 8% |
| Other | 12 | 16% |
| Unknown | 8 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 32 | 42% |
| Biochemistry, Genetics and Molecular Biology | 12 | 16% |
| Medicine and Dentistry | 9 | 12% |
| Agricultural and Biological Sciences | 7 | 9% |
| Immunology and Microbiology | 2 | 3% |
| Other | 6 | 8% |
| Unknown | 9 | 12% |