Chapter title |
Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis
|
---|---|
Chapter number | 29 |
Book title |
Inflammation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6786-5_29 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6784-1, 978-1-4939-6786-5
|
Authors |
Tae Jin Yun, Jun Seong Lee, Dahee Shim, Jae-Hoon Choi, Cheolho Cheong |
Editors |
Björn E. Clausen, Jon D. Laman |
Abstract |
Dendritic cells (DCs) are central to initiate antigen-specific immunity and tolerance. The in vivo development and distribution of DCs are now better understood even in nonlymphoid tissues [1]. Atherosclerosis is a chronic inflammatory disease of blood vessels and DCs are highly enriched in the intimal area of the aorta, which is predisposed to develop atherosclerosis. Previously, we were the first to show antigen presenting DCs and their subsets in the aorta [2, 3]. Here, we discuss several useful methods to characterize not only DCs but also other immune cells in steady state and atherosclerotic aorta. These comprise multiparameter flow cytometry strategies including intracellular staining and cell sorting, en face immunohistochemistry of DCs and regulatory T cells (Tregs), and Oil Red O staining of atherosclerotic lesions in the aorta. |
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