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Reversible site-selective labeling of membrane proteins in live cells

Overview of attention for article published in Nature Biotechnology, March 2004
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

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13 patents
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1 research highlight platform

Citations

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224 Dimensions

Readers on

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243 Mendeley
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Title
Reversible site-selective labeling of membrane proteins in live cells
Published in
Nature Biotechnology, March 2004
DOI 10.1038/nbt954
Pubmed ID
Authors

Emmanuel G Guignet, Ruud Hovius, Horst Vogel

Abstract

Chemical and biological labeling is fundamental for the elucidation of the function of proteins within biochemical cellular networks. In particular, fluorescent probes allow detection of molecular interactions, mobility and conformational changes of proteins in live cells with high temporal and spatial resolution. We present a generic method to label proteins in vivo selectively, rapidly (seconds) and reversibly, with small molecular probes that can have a wide variety of properties. These probes comprise a chromophore and a metal-ion-chelating nitrilotriacetate (NTA) moiety, which binds reversibly and specifically to engineered oligohistidine sequences in proteins of interest. We demonstrate the feasibility of the approach by binding NTA-chromophore conjugates to a representative ligand-gated ion channel and G protein-coupled receptor, each containing a polyhistidine sequence. We investigated the ionotropic 5HT(3) serotonin receptor by fluorescence measurements to characterize in vivo the probe-receptor interactions, yielding information on structure and plasma membrane distribution of the receptor.

Mendeley readers

The data shown below were compiled from readership statistics for 243 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 5 2%
United Kingdom 3 1%
United States 3 1%
Switzerland 2 <1%
France 1 <1%
Australia 1 <1%
Netherlands 1 <1%
Argentina 1 <1%
Denmark 1 <1%
Other 1 <1%
Unknown 224 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 62 26%
Researcher 61 25%
Student > Master 27 11%
Professor 20 8%
Student > Bachelor 16 7%
Other 46 19%
Unknown 11 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 100 41%
Chemistry 49 20%
Biochemistry, Genetics and Molecular Biology 34 14%
Physics and Astronomy 13 5%
Medicine and Dentistry 11 5%
Other 20 8%
Unknown 16 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 February 2020.
All research outputs
#2,698,456
of 15,625,875 outputs
Outputs from Nature Biotechnology
#3,484
of 7,078 outputs
Outputs of similar age
#2,563,799
of 14,658,373 outputs
Outputs of similar age from Nature Biotechnology
#3,458
of 7,042 outputs
Altmetric has tracked 15,625,875 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,078 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 31.6. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 14,658,373 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 7,042 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.