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Reversible site-selective labeling of membrane proteins in live cells

Overview of attention for article published in Nature Biotechnology, March 2004
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (82nd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (51st percentile)

Mentioned by

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9 patents
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1 research highlight platform

Citations

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219 Dimensions

Readers on

mendeley
237 Mendeley
Title
Reversible site-selective labeling of membrane proteins in live cells
Published in
Nature Biotechnology, March 2004
DOI 10.1038/nbt954
Pubmed ID
Authors

Emmanuel G Guignet, Ruud Hovius, Horst Vogel

Abstract

Chemical and biological labeling is fundamental for the elucidation of the function of proteins within biochemical cellular networks. In particular, fluorescent probes allow detection of molecular interactions, mobility and conformational changes of proteins in live cells with high temporal and spatial resolution. We present a generic method to label proteins in vivo selectively, rapidly (seconds) and reversibly, with small molecular probes that can have a wide variety of properties. These probes comprise a chromophore and a metal-ion-chelating nitrilotriacetate (NTA) moiety, which binds reversibly and specifically to engineered oligohistidine sequences in proteins of interest. We demonstrate the feasibility of the approach by binding NTA-chromophore conjugates to a representative ligand-gated ion channel and G protein-coupled receptor, each containing a polyhistidine sequence. We investigated the ionotropic 5HT(3) serotonin receptor by fluorescence measurements to characterize in vivo the probe-receptor interactions, yielding information on structure and plasma membrane distribution of the receptor.

Mendeley readers

The data shown below were compiled from readership statistics for 237 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 5 2%
United Kingdom 3 1%
United States 3 1%
Switzerland 2 <1%
France 1 <1%
Australia 1 <1%
Netherlands 1 <1%
Argentina 1 <1%
Denmark 1 <1%
Other 1 <1%
Unknown 218 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 62 26%
Student > Ph. D. Student 62 26%
Student > Master 26 11%
Professor 18 8%
Student > Bachelor 16 7%
Other 44 19%
Unknown 9 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 102 43%
Chemistry 48 20%
Biochemistry, Genetics and Molecular Biology 33 14%
Physics and Astronomy 13 5%
Medicine and Dentistry 10 4%
Other 19 8%
Unknown 12 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 September 2018.
All research outputs
#2,199,549
of 13,526,754 outputs
Outputs from Nature Biotechnology
#3,148
of 6,635 outputs
Outputs of similar age
#2,116,475
of 12,860,159 outputs
Outputs of similar age from Nature Biotechnology
#3,133
of 6,612 outputs
Altmetric has tracked 13,526,754 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 6,635 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 26.7. This one has gotten more attention than average, scoring higher than 51% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 12,860,159 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 6,612 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.