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Versatile Fluorescence Probes of Protein Kinase Activity

Overview of attention for article published in Journal of the American Chemical Society, November 2003
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

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Citations

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152 Dimensions

Readers on

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99 Mendeley
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Title
Versatile Fluorescence Probes of Protein Kinase Activity
Published in
Journal of the American Chemical Society, November 2003
DOI 10.1021/ja0380502
Pubmed ID
Authors

Melissa D. Shults, Barbara Imperiali

Abstract

We introduce a versatile fluorescent peptide reporter of protein kinase activity. The probe can be modified to target a desired kinase by changing the kinase recognition motif in the peptide sequence. The reporter motif contains the Sox amino acid, which generates a fluorescence signal when bound to Mg2+ present in the reaction mixture. The phosphorylated peptide exhibits a much greater affinity for Mg2+ than its unphosphorylated analogue and, thus, a greater fluorescence intensity. Product formation during phosphorylation by the kinase is easily followed by the increase in fluorescence intensity over time. These probes exhibit a 3-5-fold increase in fluorescence intensity upon phosphorylation, the magnitude of which depends on the substrate. Peptides containing the reporter functionality are phosphorylated on serine by Protein Kinase C and cAMP-dependent protein kinase and are shown to be good substrates for these enzymes. The principle of this design extends to peptides phosphorylated on threonine and tyrosine.

Mendeley readers

The data shown below were compiled from readership statistics for 99 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 2 2%
Philippines 1 1%
Denmark 1 1%
Japan 1 1%
Unknown 94 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 27 27%
Student > Master 17 17%
Researcher 14 14%
Professor > Associate Professor 13 13%
Student > Bachelor 8 8%
Other 16 16%
Unknown 4 4%
Readers by discipline Count As %
Chemistry 37 37%
Agricultural and Biological Sciences 33 33%
Biochemistry, Genetics and Molecular Biology 9 9%
Engineering 4 4%
Pharmacology, Toxicology and Pharmaceutical Science 4 4%
Other 5 5%
Unknown 7 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2018.
All research outputs
#3,298,910
of 12,875,491 outputs
Outputs from Journal of the American Chemical Society
#15,966
of 46,838 outputs
Outputs of similar age
#3,188,930
of 12,299,237 outputs
Outputs of similar age from Journal of the American Chemical Society
#15,922
of 46,729 outputs
Altmetric has tracked 12,875,491 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 46,838 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 12,299,237 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 46,729 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.