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Azacytidine induces necrosis of multiple myeloma cells through oxidative stress

Overview of attention for article published in Proteome Science, January 2013
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2 tweeters

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15 Mendeley
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Title
Azacytidine induces necrosis of multiple myeloma cells through oxidative stress
Published in
Proteome Science, January 2013
DOI 10.1186/1477-5956-11-24
Pubmed ID
Authors

Enbing Tian, Haiping Tang, Renhua Xu, Chongdong Liu, Haiteng Deng, Qingtao Wang

Abstract

Azacytidine is an inhibitor of DNA methyltransferase and is known to be an anti-leukemic agent to induce cancer cell apoptosis. In the present study, multiple myeloma cells were treated with azacytidine at clinically relevant concentrations to induce necrosis through oxidative stress. Necrotic myeloma cells exhibit unique characteristics, including enrichment of the cell-bound albumin and overexpression of endoplasmic reticulum (ER)- and mitochondrial-specific chaperones, which were not observed in other necrotic cells, including HUH-7, A2780, A549, and Hoc1a. Proteomic analysis shows that HSP60 is the most abundant up-regulated mitochondrial specific chaperone, and azacytidine-induced overexpression of HSP60 is confirmed by western blot analysis. In contrast, expression levels of cytosolic chaperones such as HSP90 and HSP71 were down-regulated in azacytidine-treated myeloma cells, concomitant with an increase of these chaperones in the cell culture medium, suggesting that mitochondrial chaperones and cytosolic chaperones behave differently in necrotic myeloma cells; ER- and mitochondrial-chaperones being retained, and cytosolic chaperones being released into the cell culture medium through the ruptured cell membrane. Our data suggest that HSP60 is potentially a new target for multiple myeloma chemotherapy.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 27%
Student > Bachelor 2 13%
Student > Ph. D. Student 2 13%
Student > Doctoral Student 2 13%
Other 1 7%
Other 4 27%
Readers by discipline Count As %
Medicine and Dentistry 8 53%
Biochemistry, Genetics and Molecular Biology 3 20%
Agricultural and Biological Sciences 2 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Unknown 1 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2013.
All research outputs
#7,814,478
of 12,503,295 outputs
Outputs from Proteome Science
#76
of 142 outputs
Outputs of similar age
#78,058
of 148,757 outputs
Outputs of similar age from Proteome Science
#1
of 1 outputs
Altmetric has tracked 12,503,295 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 142 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 148,757 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them