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Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation inE. coli

Overview of attention for article published in Biotechnology Journal, February 2017
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  • Good Attention Score compared to outputs of the same age (70th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

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3 tweeters
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1 patent

Citations

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37 Dimensions

Readers on

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71 Mendeley
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Title
Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation inE. coli
Published in
Biotechnology Journal, February 2017
DOI 10.1002/biot.201600415
Pubmed ID
Authors

Mingzhi Liang, Stefanie Frank, Heinrich Lünsdorf, Martin J. Warren, Michael B. Prentice

Abstract

Processes for the biological removal of phosphate from wastewater rely on temporary manipulation of bacterial polyphosphate levels by phased environmental stimuli. In E. coli polyphosphate levels are controlled via the polyphosphate-synthesizing enzyme polyphosphate kinase (PPK1) and exopolyphosphatases (PPX and GPPA), and are temporarily enhanced by PPK1 overexpression and reduced by PPX overexpression. We hypothesised that partitioning PPK1 from cytoplasmic exopolyphosphatases would increase and stabilise E. coli polyphosphate levels. Partitioning was achieved by co-expression of E. coli PPK1 fused with a microcompartment-targeting sequence and an artificial operon of Citrobacter freundii bacterial microcompartment genes. Encapsulation of targeted PPK1 resulted in persistent phosphate uptake and stably increased cellular polyphosphate levels throughout cell growth and into the stationary phase, while PPK1 overexpression alone produced temporary polyphosphate increase and phosphate uptake. Targeted PPK1 increased polyphosphate in microcompartments 8-fold compared with non-targeted PPK1. Co-expression of PPX polyphosphatase with targeted PPK1 had little effect on elevated cellular polyphosphate levels because microcompartments retained polyphosphate. Co-expression of PPX with non-targeted PPK1 reduced cellular polyphosphate levels. Thus, subcellular compartmentalisation of a polymerising enzyme sequesters metabolic products from competing catabolism by preventing catabolic enzyme access. Specific application of this process to polyphosphate is of potential application for biological phosphate removal.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
China 1 1%
Singapore 1 1%
Unknown 68 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 24%
Researcher 14 20%
Student > Master 11 15%
Student > Doctoral Student 6 8%
Other 6 8%
Other 10 14%
Unknown 7 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 35 49%
Agricultural and Biological Sciences 12 17%
Chemistry 5 7%
Medicine and Dentistry 2 3%
Chemical Engineering 1 1%
Other 3 4%
Unknown 13 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2019.
All research outputs
#4,597,023
of 17,135,529 outputs
Outputs from Biotechnology Journal
#663
of 1,511 outputs
Outputs of similar age
#105,374
of 364,693 outputs
Outputs of similar age from Biotechnology Journal
#25
of 46 outputs
Altmetric has tracked 17,135,529 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 1,511 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 364,693 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.