Title |
A light in the shadow: the use of Lucifer Yellow technique to demonstrate nectar reabsorption
|
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Published in |
Plant Methods, June 2013
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DOI | 10.1186/1746-4811-9-20 |
Pubmed ID | |
Authors |
Poliana Cardoso-Gustavson, João Marcelo Robazzi Bignelli Valente Aguiar, Emerson Ricardo Pansarin, Fábio de Barros |
Abstract |
Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. Although this method works perfectly, it is complex and requires specific supplies and equipment. Therefore, here we propose an efficient method to obtain a visual demonstration of nectar reabsorption, adapting the use of Lucifer Yellow CH (LYCH), a fluorescent membrane-impermeable dye that can enter the vacuole by endocytosis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 67% |
Spain | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 2 | 6% |
Mexico | 1 | 3% |
Unknown | 30 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 27% |
Student > Master | 6 | 18% |
Student > Bachelor | 4 | 12% |
Researcher | 4 | 12% |
Student > Doctoral Student | 2 | 6% |
Other | 3 | 9% |
Unknown | 5 | 15% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 19 | 58% |
Biochemistry, Genetics and Molecular Biology | 2 | 6% |
Environmental Science | 1 | 3% |
Medicine and Dentistry | 1 | 3% |
Chemistry | 1 | 3% |
Other | 1 | 3% |
Unknown | 8 | 24% |