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Compartmentalized Culture of Perivascular Stroma and Endothelial Cells in a Microfluidic Model of the Human Endometrium

Overview of attention for article published in Annals of Biomedical Engineering, January 2017
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Mentioned by

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2 tweeters

Citations

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12 Dimensions

Readers on

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39 Mendeley
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Title
Compartmentalized Culture of Perivascular Stroma and Endothelial Cells in a Microfluidic Model of the Human Endometrium
Published in
Annals of Biomedical Engineering, January 2017
DOI 10.1007/s10439-017-1797-5
Pubmed ID
Authors

Juan S. Gnecco, Virginia Pensabene, David J. Li, Tianbing Ding, Elliot E. Hui, Kaylon L. Bruner-Tran, Kevin G. Osteen

Abstract

The endometrium is the inner lining of the uterus. Following specific cyclic hormonal stimulation, endometrial stromal fibroblasts (stroma) and vascular endothelial cells exhibit morphological and biochemical changes to support embryo implantation and regulate vascular function, respectively. Herein, we integrated a resin-based porous membrane in a dual chamber microfluidic device in polydimethylsiloxane that allows long term in vitro co-culture of human endometrial stromal and endothelial cells. This transparent, 2-μm porous membrane separates the two chambers, allows for the diffusion of small molecules and enables high resolution bright field and fluorescent imaging. Within our primary human co-culture model of stromal and endothelial cells, we simulated the temporal hormone changes occurring during an idealized 28-day menstrual cycle. We observed the successful differentiation of stroma into functional decidual cells, determined by morphology as well as biochemically as measured by increased production of prolactin. By controlling the microfluidic properties of the device, we additionally found that shear stress forces promoted cytoskeleton alignment and tight junction formation in the endothelial layer. Finally, we demonstrated that the endometrial perivascular stroma model was sustainable for up to 4 weeks, remained sensitive to steroids and is suitable for quantitative biochemical analysis. Future utilization of this device will allow the direct evaluation of paracrine and endocrine crosstalk between these two cell types as well as studies of immunological events associated with normal vs. disease-related endometrial microenvironments.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
Unknown 38 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 28%
Researcher 8 21%
Unspecified 7 18%
Student > Doctoral Student 5 13%
Other 4 10%
Other 4 10%
Readers by discipline Count As %
Unspecified 11 28%
Medicine and Dentistry 7 18%
Engineering 6 15%
Biochemistry, Genetics and Molecular Biology 6 15%
Agricultural and Biological Sciences 5 13%
Other 4 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2018.
All research outputs
#7,608,285
of 13,205,256 outputs
Outputs from Annals of Biomedical Engineering
#888
of 1,327 outputs
Outputs of similar age
#162,801
of 344,686 outputs
Outputs of similar age from Annals of Biomedical Engineering
#18
of 36 outputs
Altmetric has tracked 13,205,256 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,327 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,686 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.