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Evidence for Intramolecular Antiparallel Beta-Sheet Structure in Alpha-Synuclein Fibrils from a Combination of Two-Dimensional Infrared Spectroscopy and Atomic Force Microscopy

Overview of attention for article published in Scientific Reports, January 2017
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (91st percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

news
3 news outlets

Citations

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111 Dimensions

Readers on

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191 Mendeley
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Title
Evidence for Intramolecular Antiparallel Beta-Sheet Structure in Alpha-Synuclein Fibrils from a Combination of Two-Dimensional Infrared Spectroscopy and Atomic Force Microscopy
Published in
Scientific Reports, January 2017
DOI 10.1038/srep41051
Pubmed ID
Authors

Steven J. Roeters, Aditya Iyer, Galja Pletikapić, Vladimir Kogan, Vinod Subramaniam, Sander Woutersen

Abstract

The aggregation of the intrinsically disordered protein alpha-synuclein (αS) into amyloid fibrils is thought to play a central role in the pathology of Parkinson's disease. Using a combination of techniques (AFM, UV-CD, XRD, and amide-I 1D- and 2D-IR spectroscopy) we show that the structure of αS fibrils varies as a function of ionic strength: fibrils aggregated in low ionic-strength buffers ([NaCl] ≤ 25 mM) have a significantly different structure than fibrils grown in higher ionic-strength buffers. The observations for fibrils aggregated in low-salt buffers are consistent with an extended conformation of αS molecules, forming hydrogen-bonded intermolecular β-sheets that are loosely packed in a parallel fashion. For fibrils aggregated in high-salt buffers (including those prepared in buffers with a physiological salt concentration) the measurements are consistent with αS molecules in a more tightly-packed, antiparallel intramolecular conformation, and suggest a structure characterized by two twisting stacks of approximately five hydrogen-bonded intermolecular β-sheets each. We find evidence that the high-frequency peak in the amide-I spectrum of αS fibrils involves a normal mode that differs fundamentally from the canonical high-frequency antiparallel β-sheet mode. The high sensitivity of the fibril structure to the ionic strength might form the basis of differences in αS-related pathologies.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 191 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 <1%
Unknown 190 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 51 27%
Researcher 25 13%
Student > Master 21 11%
Student > Bachelor 20 10%
Student > Doctoral Student 8 4%
Other 29 15%
Unknown 37 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 47 25%
Chemistry 30 16%
Agricultural and Biological Sciences 16 8%
Neuroscience 16 8%
Materials Science 8 4%
Other 26 14%
Unknown 48 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 21. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2017.
All research outputs
#1,496,535
of 22,947,506 outputs
Outputs from Scientific Reports
#14,184
of 123,897 outputs
Outputs of similar age
#34,171
of 419,069 outputs
Outputs of similar age from Scientific Reports
#495
of 3,831 outputs
Altmetric has tracked 22,947,506 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 123,897 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.2. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 419,069 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 3,831 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.