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Brassinosteroids

Overview of attention for book
Cover of 'Brassinosteroids'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues
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    Chapter 2 Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
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    Chapter 3 Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice
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    Chapter 4 Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness
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    Chapter 5 Approaches to Study Light Effects on Brassinosteroid Sensitivity
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    Chapter 6 A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity
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    Chapter 7 Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing
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    Chapter 8 Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA
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    Chapter 9 Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development
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    Chapter 10 Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells
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    Chapter 11 Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay
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    Chapter 12 Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry
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    Chapter 13 Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment
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    Chapter 14 Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation
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    Chapter 15 The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops
  17. Altmetric Badge
    Chapter 16 Brassinosteroid Action in Plant Abiotic Stress Tolerance
Attention for Chapter 1: Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues
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Chapter title
Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues
Chapter number 1
Book title
Brassinosteroids
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6813-8_1
Pubmed ID
Book ISBNs
978-1-4939-6811-4, 978-1-4939-6813-8
Authors

Danuše Tarkowská, Miroslav Strnad

Editors

Eugenia Russinova, Ana I. Caño-Delgado

Abstract

Brassinosteroids (BRs) are a class of steroid plant hormones that participate with other plant hormones in the regulation of numerous developmental processes, including root and shoot growth, vascular differentiation, fertility, and seed germination. A characteristic feature of all plant hormones, including BRs, is that their concentration is extremely low in plant tissues and, therefore, the methods dealing with their determination belong to ultra-trace analysis, for which very sensitive analytical tools are needed. The analysis of natural BRs is essential when their functions and roles in plant growth and development are to be elucidated. Here, we describe a reliable protocol for high-throughput extraction and purification of BRs. The procedure consists of two solid-phase extraction steps and provides selective enrichment and efficient cleanup of these compounds from complex plant extracts. The protocol is designed for sensitive liquid chromatography-tandem mass spectrometry-based method for simultaneous detection of 22 naturally occurring BRs, including their biosynthetic precursors and most of their biologically active metabolites, without need for derivatization.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 38%
Professor 1 13%
Researcher 1 13%
Student > Master 1 13%
Unknown 2 25%
Readers by discipline Count As %
Chemistry 2 25%
Agricultural and Biological Sciences 1 13%
Biochemistry, Genetics and Molecular Biology 1 13%
Unknown 4 50%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2018.
All research outputs
#9,968,429
of 12,452,101 outputs
Outputs from Methods in molecular biology
#4,219
of 8,342 outputs
Outputs of similar age
#233,729
of 332,512 outputs
Outputs of similar age from Methods in molecular biology
#758
of 1,475 outputs
Altmetric has tracked 12,452,101 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,342 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,475 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.