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Brassinosteroids

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Cover of 'Brassinosteroids'

Table of Contents

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    Book Overview
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    Chapter 1 Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues
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    Chapter 2 Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
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    Chapter 3 Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice
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    Chapter 4 Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness
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    Chapter 5 Approaches to Study Light Effects on Brassinosteroid Sensitivity
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    Chapter 6 A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity
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    Chapter 7 Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing
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    Chapter 8 Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA
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    Chapter 9 Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development
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    Chapter 10 Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells
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    Chapter 11 Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay
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    Chapter 12 Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry
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    Chapter 13 Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment
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    Chapter 14 Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation
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    Chapter 15 The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops
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    Chapter 16 Brassinosteroid Action in Plant Abiotic Stress Tolerance
Attention for Chapter 4: Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness
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Chapter title
Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness
Chapter number 4
Book title
Brassinosteroids
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6813-8_4
Pubmed ID
Book ISBNs
978-1-4939-6811-4, 978-1-4939-6813-8
Authors

Claudia Corvalán, Sunghwa Choe

Editors

Eugenia Russinova, Ana I. Caño-Delgado

Abstract

Environmental conditions can affect stability of proteins at transcriptional or posttranscriptional levels to modulate their functions. Here we describe a method to observe changes in protein stability under different light conditions. In brief, Arabidopsis thaliana seedlings were maintained under various light regimes from continuous light to total darkness or transitions from light to dark, whereafter total protein was extracted from plants. Proteins were measured and resolved on sodium dodecyl sulfate-polyacrylamide gels and transferred to polyvinylidene difluoride membranes. Blots were incubated with the corresponding antibodies for the visualization of protein bands. The protocol described has been successfully applied in wild-type, different transgenic, and mutant background plants to study how light alone or in combination with other factors influences protein stability.

Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 100%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 100%