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Brassinosteroids

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Cover of 'Brassinosteroids'

Table of Contents

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    Book Overview
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    Chapter 1 Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues
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    Chapter 2 Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
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    Chapter 3 Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice
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    Chapter 4 Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness
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    Chapter 5 Approaches to Study Light Effects on Brassinosteroid Sensitivity
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    Chapter 6 A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity
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    Chapter 7 Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing
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    Chapter 8 Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA
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    Chapter 9 Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development
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    Chapter 10 Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells
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    Chapter 11 Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay
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    Chapter 12 Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry
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    Chapter 13 Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment
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    Chapter 14 Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation
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    Chapter 15 The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops
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    Chapter 16 Brassinosteroid Action in Plant Abiotic Stress Tolerance
Attention for Chapter 14: Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation
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Chapter title
Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation
Chapter number 14
Book title
Brassinosteroids
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6813-8_14
Pubmed ID
Book ISBNs
978-1-4939-6811-4, 978-1-4939-6813-8
Authors

Sara Martins, Grégory Vert, Yvon Jaillais

Editors

Eugenia Russinova, Ana I. Caño-Delgado

Abstract

Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Philippines 1 17%
Unknown 5 83%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 33%
Researcher 2 33%
Student > Bachelor 1 17%
Professor > Associate Professor 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 67%
Biochemistry, Genetics and Molecular Biology 2 33%