| Chapter title |
Approaches to Study Light Effects on Brassinosteroid Sensitivity
|
|---|---|
| Chapter number | 5 |
| Book title |
Brassinosteroids
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6813-8_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6811-4, 978-1-4939-6813-8
|
| Authors |
Sandi Paulišić, Maria José Molina-Contreras, Irma Roig-Villanova, Jaime F. Martínez-García |
| Editors |
Eugenia Russinova, Ana I. Caño-Delgado |
| Abstract |
Light perception and hormone signaling in plants are likely connected at multiple points. Light conditions, perceived by photoreceptors, control plant responses by altering hormone concentration, tissue sensitivity, or a combination of both. Whereas it is relatively straightforward to assess the light effects on hormone levels, hormone sensitivity is subjected to interpretation. In Arabidopsis thaliana seedlings, hypocotyl length is strongly affected by light conditions. As hypocotyl elongation also depends on brassinosteroids (BRs), assaying this response provides a valuable and easy way to measure the responsiveness of seedlings to BRs and the impact of light. We describe a simple protocol to evaluate the responsiveness of hypocotyls to commercial BRs and/or BR inhibitors under a range of light conditions. These assays can be used to establish whether light affects BR sensitivity or whether BRs affect light sensitivity. Overall, our protocol can be easily applied for deetiolation (under polychromatic or monochromatic light) and simulated shade treatments combined with BR treatments. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Student > Ph. D. Student | 1 | 17% |
| Professor > Associate Professor | 1 | 17% |
| Researcher | 1 | 17% |
| Student > Doctoral Student | 1 | 17% |
| Other | 0 | 0% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 33% |
| Unspecified | 1 | 17% |
| Nursing and Health Professions | 1 | 17% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Unknown | 1 | 17% |