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Chapter title |
Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation
|
---|---|
Chapter number | 14 |
Book title |
Brassinosteroids
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6813-8_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6811-4, 978-1-4939-6813-8
|
Authors |
Sara Martins, Grégory Vert, Yvon Jaillais |
Editors |
Eugenia Russinova, Ana I. Caño-Delgado |
Abstract |
Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Philippines | 1 | 14% |
Unknown | 6 | 86% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 43% |
Student > Master | 2 | 29% |
Student > Bachelor | 1 | 14% |
Professor > Associate Professor | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 71% |
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Engineering | 1 | 14% |