Title |
Evaluation of a real-time quantitative PCR to measure the wild Plasmodium falciparum infectivity rate in salivary glands of Anopheles gambiae
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Published in |
Malaria Journal, July 2013
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DOI | 10.1186/1475-2875-12-224 |
Pubmed ID | |
Authors |
Alexandra Marie, Anne Boissière, Majoline Tchioffo Tsapi, Anne Poinsignon, Parfait H Awono-Ambéné, Isabelle Morlais, Franck Remoue, Sylvie Cornelie |
Abstract |
Evaluation of malaria sporozoite rates in the salivary glands of Anopheles gambiae is essential for estimating the number of infective mosquitoes, and consequently, the entomological inoculation rate (EIR). EIR is a key indicator for evaluating the risk of malaria transmission. Although the enzyme-linked immunosorbent assay specific for detecting the circumsporozoite protein (CSP-ELISA) is routinely used in the field, it presents several limitations. A multiplex PCR can also be used to detect the four species of Plasmodium in salivary glands. The aim of this study was to evaluate the efficacy of a real-time quantitative PCR in detecting and quantifying wild Plasmodium falciparum in the salivary glands of An. gambiae. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | <1% |
United States | 1 | <1% |
Madagascar | 1 | <1% |
Unknown | 100 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 23 | 22% |
Student > Master | 23 | 22% |
Researcher | 21 | 20% |
Student > Bachelor | 7 | 7% |
Other | 4 | 4% |
Other | 13 | 13% |
Unknown | 12 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 41 | 40% |
Biochemistry, Genetics and Molecular Biology | 13 | 13% |
Medicine and Dentistry | 9 | 9% |
Immunology and Microbiology | 7 | 7% |
Environmental Science | 6 | 6% |
Other | 11 | 11% |
Unknown | 16 | 16% |