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Breed and adaptive response modulate bovine peripheral blood cells’ transcriptome

Overview of attention for article published in Journal of Animal Science and Biotechnology, January 2017
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Title
Breed and adaptive response modulate bovine peripheral blood cells’ transcriptome
Published in
Journal of Animal Science and Biotechnology, January 2017
DOI 10.1186/s40104-017-0143-y
Pubmed ID
Authors

Nataliya Pošćić, Tommaso Montanari, Mariasilvia D’Andrea, Danilo Licastro, Fabio Pilla, Paolo Ajmone-Marsan, Andrea Minuti, Sandy Sgorlon

Abstract

Adaptive response includes a variety of physiological modifications to face changes in external or internal conditions and adapt to a new situation. The acute phase proteins (APPs) are reactants synthesized against environmental stimuli like stress, infection, inflammation. To delineate the differences in molecular constituents of adaptive response to the environment we performed the whole-blood transcriptome analysis in Italian Holstein (IH) and Italian Simmental (IS) breeds. For this, 663 IH and IS cows from six commercial farms were clustered according to the blood level of APPs. Ten extreme individuals (five APP+ and APP- variants) from each farm were selected for the RNA-seq using the Illumina sequencing technology. Differentially expressed (DE) genes were analyzed using dynamic impact approach (DIA) and DAVID annotation clustering. Milk production data were statistically elaborated to assess the association of APP+ and APP- gene expression patterns with variations in milk parameters. The overall de novo assembly of cDNA sequence data generated 13,665 genes expressed in bovine blood cells. Comparative genomic analysis revealed 1,152 DE genes in the comparison of all APP+ vs. all APP- variants; 531 and 217 DE genes specific for IH and IS comparison respectively. In all comparisons overexpressed genes were more represented than underexpressed ones. DAVID analysis revealed 369 DE genes across breeds, 173 and 73 DE genes in IH and IS comparison respectively. Among the most impacted pathways for both breeds were vitamin B6 metabolism, folate biosynthesis, nitrogen metabolism and linoleic acid metabolism. Both DIA and DAVID approaches produced a high number of significantly impacted genes and pathways with a narrow connection to adaptive response in cows with high level of blood APPs. A similar variation in gene expression and impacted pathways between APP+ and APP- variants was found between two studied breeds. Such similarity was also confirmed by annotation clustering of the DE genes. However, IH breed showed higher and more differentiated impacts compared to IS breed and such particular features in the IH adaptive response could be explained by its higher metabolic activity. Variations of milk production data were significantly associated with APP+ and APP- gene expression patterns.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 3 27%
Student > Ph. D. Student 2 18%
Student > Master 2 18%
Student > Doctoral Student 1 9%
Student > Bachelor 1 9%
Other 2 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 45%
Unspecified 4 36%
Biochemistry, Genetics and Molecular Biology 1 9%
Veterinary Science and Veterinary Medicine 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2017.
All research outputs
#11,170,233
of 12,554,960 outputs
Outputs from Journal of Animal Science and Biotechnology
#249
of 273 outputs
Outputs of similar age
#279,812
of 338,866 outputs
Outputs of similar age from Journal of Animal Science and Biotechnology
#5
of 6 outputs
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