↓ Skip to main content
What is this page? Embed badge

Haplotyping

Overview of attention for book
Cover of 'Haplotyping'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Haplotyping of Heterozygous SNPs in Genomic DNA Using Long-Range PCR.
  3. Altmetric Badge
    Chapter 2 Quantification and Sequencing of Crossover Recombinant Molecules from Arabidopsis Pollen DNA.
  4. Altmetric Badge
    Chapter 3 PacBio for Haplotyping in Gene Families
  5. Altmetric Badge
    Chapter 4 High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes
  6. Altmetric Badge
    Chapter 5 High-Throughput Sequencing of the Major Histocompatibility Complex following Targeted Sequence Capture
  7. Altmetric Badge
    Chapter 6 Pedigree-Defined Haplotypes and Their Applications to Genetic Studies
  8. Altmetric Badge
    Chapter 7 Haplotyping a Non-meiotic Diploid Fungal Pathogen Using Induced Aneuploidies and SNP/CGH Microarray Analysis.
  9. Altmetric Badge
    Chapter 8 Whole-Genome Haplotyping of Single Sperm of Daphnia pulex (Crustacea, Anomopoda).
  10. Altmetric Badge
    Chapter 9 Chromosome-Range Whole-Genome High-Throughput Experimental Haplotyping by Single-Chromosome Microdissection
  11. Altmetric Badge
    Chapter 10 Phased Genome Sequencing Through Chromosome Sorting
  12. Altmetric Badge
    Chapter 11 Long Fragment Read (LFR) Technology: Cost-Effective, High-Quality Genome-Wide Molecular Haplotyping
  13. Altmetric Badge
    Chapter 12 Contiguity-Preserving Transposition Sequencing (CPT-Seq) for Genome-Wide Haplotyping, Assembly, and Single-Cell ATAC-Seq
  14. Altmetric Badge
    Chapter 13 A Fosmid Pool-Based Next Generation Sequencing Approach to Haplotype-Resolve Whole Genomes
  15. Altmetric Badge
    Chapter 14 Discovery of Rare Haplotypes by Typing Millions of Single-Molecules with Bead Emulsion Haplotyping (BEH)
  16. Altmetric Badge
    Chapter 15 Computational Haplotype Inference from Pooled Samples
Attention for Chapter 3: PacBio for Haplotyping in Gene Families
Altmetric Badge

Mentioned by

twitter
1 X user

Readers on

mendeley
6 Mendeley
Summary X
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
PacBio for Haplotyping in Gene Families
Chapter number 3
Book title
Haplotyping
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6750-6_3
Pubmed ID
28138840
Book ISBNs
978-1-4939-6748-3, 978-1-4939-6750-6
Authors

Wei Zhang, Joachim Messing, Zhang, Wei, Messing, Joachim

Editors

Irene Tiemann-Boege, Andrea Betancourt

Abstract

The throughput and read length provided by Pacific Bioscience (PacBio) Single Molecule Real Time (SMRT) sequencing platform makes it feasible to construct contiguous, non-chimeric sequences. This is especially useful for genes with repetitive sequences in their gene bodies in gene families. We illustrate the use of PacBio to sequence and assemble hundreds of transcripts of gluten gene families from different cultivars of wheat using sequence from a single SMRT cell. To this end, we barcoded amplicons from different cultivars, then pooled these into one library for sequencing. Sequencing reads were later separated by the barcodes and further sorted into different gene groups by blast. The reads from each gene are then assembled by SeqmanNGen software. Given the length of 1 kb for each sequence derived from an initial molecule, the phase of the polymorphisms is not lost and can be used to infer also haplotype differences between different cultivars.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Other 1 17%
Student > Bachelor 1 17%
Professor 1 17%
Student > Ph. D. Student 1 17%
Student > Master 1 17%
Other 0 0%
Unknown 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 67%
Computer Science 1 17%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 July 2018.
All research outputs
#20,402,251
of 22,952,268 outputs
Outputs from Methods in molecular biology
#9,921
of 13,137 outputs
Outputs of similar age
#355,930
of 420,204 outputs
Outputs of similar age from Methods in molecular biology
#913
of 1,167 outputs
Altmetric has tracked 22,952,268 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,137 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,204 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,167 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.