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Hypertension

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Cover of 'Hypertension'

Table of Contents

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    Book Overview
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    Chapter 1 Hypertension
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    Chapter 2 Methods to Assess Genetic Risk Prediction
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    Chapter 3 Microarray Analysis of Hypertension
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    Chapter 4 Tissue Proteomics in Vascular Disease
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    Chapter 5 Urine Metabolomics in Hypertension Research
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    Chapter 6 Systems Biology Approach in Hypertension Research
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    Chapter 7 Measurement of Angiotensin Peptides: HPLC-RIA
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    Chapter 8 Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2)
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    Chapter 9 Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate
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    Chapter 10 Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-Chip/Mass Spectrometry, and Functional Assays
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    Chapter 11 Analysis of the Aldosterone Synthase (CYP11B2) and 11β-Hydroxylase (CYP11B1) Genes
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    Chapter 12 Dopaminergic Immunofluorescence Studies in Kidney Tissue
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    Chapter 13 Techniques for the Evaluation of the Genetic Expression, Intracellular Storage, and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (NPs)
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    Chapter 14 Intracellular Free Calcium Measurement Using Confocal Imaging
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    Chapter 15 Measuring T-Type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells
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    Chapter 16 In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs
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    Chapter 17 In Vitro and In Vivo Approaches to Assess Rho Kinase Activity
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    Chapter 18 NADPH Oxidases and Measurement of Reactive Oxygen Species
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    Chapter 19 Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation
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    Chapter 20 Assessment of Caveolae/Lipid Rafts in Isolated Cells
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    Chapter 21 Isolation and Characterization of Circulating Microparticles by Flow Cytometry
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    Chapter 22 Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR
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    Chapter 23 Isolation and Differentiation of Murine Macrophages
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    Chapter 24 Isolation and Differentiation of Human Macrophages
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    Chapter 25 Isolation of Immune Cells for Adoptive Transfer
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    Chapter 26 Isolation and Culture of Endothelial Cells from Large Vessels
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    Chapter 27 Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels
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    Chapter 28 Evaluation of Endothelial Dysfunction In Vivo
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    Chapter 29 Vascular Reactivity of Isolated Aorta to Study the Angiotensin-(1-7) Actions
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    Chapter 30 Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of PPARγ
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    Chapter 31 Renal Delivery of Anti-microRNA Oligonucleotides in Rats
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    Chapter 32 In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation, and Assessment of Physiologic Outputs
Attention for Chapter 12: Dopaminergic Immunofluorescence Studies in Kidney Tissue
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Chapter title
Dopaminergic Immunofluorescence Studies in Kidney Tissue
Chapter number 12
Book title
Hypertension
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6625-7_12
Pubmed ID
Book ISBNs
978-1-4939-6623-3, 978-1-4939-6625-7
Authors

J. J. Gildea, R. E. Van Sciver, H. E. McGrath, B. A. Kemp, P. A. Jose, R. M. Carey, R. A. Felder

Editors

Rhian M. Touyz, Ernesto L. Schiffrin

Abstract

The kidney is a highly integrated system of specialized differentiated cells that are responsible for fluid and electrolyte balance in the body. While much of today's research focuses on isolated nephron segments or cells from nephron segments grown in tissue culture, an often overlooked technique that can provide a unique view of many cell types in the kidney is slice culture. Here, we describe techniques that use freshly excised kidney tissue from rats to perform a variety of experiments shortly after isolating the tissue. By slicing the rat kidney in a "bread loaf" format, multiple studies can be performed on slices from the same tissue in parallel. Cryosectioning and staining of the tissue allow for the evaluation of physiological or biochemical responses in a wide variety of specific nephron segments. The procedures described within this chapter can also be extended to human or mouse kidney tissue.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 50%
Unknown 1 50%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 50%
Professor > Associate Professor 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Agricultural and Biological Sciences 1 50%