Chapter title |
Isolation and Differentiation of Murine Macrophages
|
---|---|
Chapter number | 23 |
Book title |
Hypertension
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6625-7_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6623-3, 978-1-4939-6625-7
|
Authors |
Francisco J. Rios Ph.D., Rhian M. Touyz, Augusto C. Montezano, Francisco J. Rios |
Editors |
Rhian M. Touyz, Ernesto L. Schiffrin |
Abstract |
Macrophages play a major role in inflammation, wound healing, and tissue repair. Infiltrated monocytes differentiate into different macrophage subtypes with protective or pathogenic activities in vascular lesions. In the heart and vascular tissues, pathological activation promotes cardiovascular inflammation and remodeling and there is increasing evidence that macrophages play important mechanisms in this environment. Primary murine macrophages can be obtained from: bone marrow by different treatments (granulocyte-macrophage colony-stimulating factor-GM-CSF, macrophage colony-stimulating factor-M-CSF or supernatant of murine fibroblast L929), peritoneal cavity (resident or thioglycolate elicit macrophages), from the lung (alveolar macrophages) or from adipose tissue. In this chapter we describe some protocols to obtain primary murine macrophages and how to identify a pure macrophage population or activation phenotypes using different markers. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 104 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 15 | 14% |
Student > Ph. D. Student | 12 | 12% |
Student > Bachelor | 11 | 11% |
Student > Doctoral Student | 9 | 9% |
Student > Master | 7 | 7% |
Other | 15 | 14% |
Unknown | 35 | 34% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 14 | 13% |
Biochemistry, Genetics and Molecular Biology | 13 | 13% |
Immunology and Microbiology | 10 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 8 | 8% |
Neuroscience | 5 | 5% |
Other | 17 | 16% |
Unknown | 37 | 36% |