| Chapter title |
Evaluation of Endothelial Dysfunction In Vivo
|
|---|---|
| Chapter number | 28 |
| Book title |
Hypertension
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6625-7_28 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6623-3, 978-1-4939-6625-7
|
| Authors |
Mihail Todiras, Natalia Alenina, Michael Bader |
| Editors |
Rhian M. Touyz, Ernesto L. Schiffrin |
| Abstract |
Vascular endothelial cells play a major role in maintaining cardiovascular homeostasis. Impairment of physiological properties of the endothelium, such as the promotion of vasodilation and anti-aggregation, leads to a condition called endothelial dysfunction. Endothelial dysfunction is an important early event in the pathogenesis of atherosclerosis and has been shown to have prognostic value in predicting vascular events including stroke and myocardial infarction.Endothelial-dependent vasodilation is one of the most widely used methods for assessment of endothelial function in rodents. It includes pharmacological stimulation (for example by acetylcholine) of endothelial release of NO and other vasoactive compounds in comparison with vascular response to endothelium-independent dilators such as sodium nitroprusside. However, usually this technique is performed in anesthetized animals. Here we describe a method which allows evaluation of endothelial dysfunction in conscious, freely moving mice and rats. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 24% |
| Student > Bachelor | 2 | 12% |
| Professor | 2 | 12% |
| Unspecified | 1 | 6% |
| Other | 1 | 6% |
| Other | 2 | 12% |
| Unknown | 5 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 18% |
| Agricultural and Biological Sciences | 2 | 12% |
| Medicine and Dentistry | 2 | 12% |
| Nursing and Health Professions | 1 | 6% |
| Unspecified | 1 | 6% |
| Other | 2 | 12% |
| Unknown | 6 | 35% |