Chapter title |
Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach
|
---|---|
Chapter number | 3 |
Book title |
Proteomics
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6747-6_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6745-2, 978-1-4939-6747-6
|
Authors |
Sophie L. Kan, Nehmé Saksouk, Jérome Déjardin |
Editors |
Lucio Comai, Jonathan E. Katz, Parag Mallick |
Abstract |
The biological functions of given genomic regions are ruled by the local chromatin composition. The Proteomics of Isolated Chromatin segments approach (PICh) is a powerful and unbiased method to analyze the composition of chosen chromatin segments, provided they are abundant (repeated) or that the organism studied has a small genome. PICh can be used to identify novel and unexpected regulatory factors, or when combined with quantitative mass spectrometric approaches, to characterize the function of a defined factor at the chosen locus, by quantifying composition changes at the locus upon removal/addition of that factor. |
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