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Proteomics

Overview of attention for book
Cover of 'Proteomics'

Table of Contents

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    Book Overview
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    Chapter 1 A Robust Protocol for Protein Extraction and Digestion
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    Chapter 2 Improving Proteome Coverage and Sample Recovery with Enhanced FASP (eFASP) for Quantitative Proteomic Experiments
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    Chapter 3 Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach
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    Chapter 4 Profiling Cell Lines Nuclear Sub-proteome
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    Chapter 5 Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography
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    Chapter 6 Full Membrane Protein Coverage Digestion and Quantitative Bottom-Up Mass Spectrometry Proteomics
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    Chapter 7 Hydrophilic Strong Anion Exchange (hSAX) Chromatography Enables Deep Fractionation of Tissue Proteomes
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    Chapter 8 High pH Reversed-Phase Micro-Columns for Simple, Sensitive, and Efficient Fractionation of Proteome and (TMT labeled) Phosphoproteome Digests
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    Chapter 9 Multi-Lectin Affinity Chromatography for Separation, Identification, and Quantitation of Intact Protein Glycoforms in Complex Biological Mixtures
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    Chapter 10 Parallel Exploration of Interaction Space by BioID and Affinity Purification Coupled to Mass Spectrometry
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    Chapter 11 LUMIER: A Discovery Tool for Mammalian Protein Interaction Networks
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    Chapter 12 Dual-Color, Multiplex Analysis of Protein Microarrays for Precision Medicine
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    Chapter 13 Quantitative Proteomics Using SILAC
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    Chapter 14 Relative Protein Quantification Using Tandem Mass Tag Mass Spectrometry
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    Chapter 15 Pathway-Informed Discovery and Targeted Proteomic Workflows Using Mass Spectrometry
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    Chapter 16 Generation of High-Quality SWATH® Acquisition Data for Label-free Quantitative Proteomics Studies Using TripleTOF® Mass Spectrometers
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    Chapter 17 Annotating Mutational Effects on Proteins and Protein Interactions: Designing Novel and Revisiting Existing Protocols
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    Chapter 18 Protein Micropatterning Assay: Quantitative Analysis of Protein–Protein Interactions
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    Chapter 19 Designing Successful Proteomics Experiments
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    Chapter 20 Automated SWATH Data Analysis Using Targeted Extraction of Ion Chromatograms
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    Chapter 21 Virtualization of Legacy Instrumentation Control Computers for Improved Reliability, Operational Life, and Management
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    Chapter 22 Statistical Assessment of QC Metrics on Raw LC-MS/MS Data
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    Chapter 23 Data Conversion with ProteoWizard msConvert
Attention for Chapter 6: Full Membrane Protein Coverage Digestion and Quantitative Bottom-Up Mass Spectrometry Proteomics
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Chapter title
Full Membrane Protein Coverage Digestion and Quantitative Bottom-Up Mass Spectrometry Proteomics
Chapter number 6
Book title
Proteomics
Published in
Methods in molecular biology, February 2017
DOI 10.1007/978-1-4939-6747-6_6
Pubmed ID
Book ISBNs
978-1-4939-6745-2, 978-1-4939-6747-6
Authors

Joseph Capri, Julian P. Whitelegge Ph.D., Julian P. Whitelegge

Editors

Lucio Comai, Jonathan E. Katz, Parag Mallick

Abstract

A true and accurate bottom-up global proteomic measurement will only be achieved when all proteins in a sample can be digested efficiently and at least some peptides recovered on which to base an estimate of abundance. Integral membrane proteins make up around one-third of the proteome and require specialized protocols if they are to be successfully solubilized for efficient digestion by the enzymes used in bottom-up proteomics. The protocol described relies upon solubilization using the detergents sodium deoxycholate and lauryl sarcosine with heating to 95 °C. A subset of peptides is purified by reverse-phase solid-phase extraction and fractionated by strong-cation exchange prior to nano-liquid chromatography with data-dependent tandem mass spectrometry. For quantitative proteomics experiments a protocol is described for stable-isotope coding of peptides using dimethylation of primary amines allowing for three-way sample multiplexing.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 38%
Student > Bachelor 2 15%
Other 1 8%
Researcher 1 8%
Professor > Associate Professor 1 8%
Other 0 0%
Unknown 3 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 23%
Chemistry 2 15%
Agricultural and Biological Sciences 2 15%
Nursing and Health Professions 1 8%
Medicine and Dentistry 1 8%
Other 1 8%
Unknown 3 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 February 2017.
All research outputs
#20,406,219
of 22,955,959 outputs
Outputs from Methods in molecular biology
#9,919
of 13,137 outputs
Outputs of similar age
#359,219
of 424,210 outputs
Outputs of similar age from Methods in molecular biology
#971
of 1,241 outputs
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