| Chapter title |
Protein Micropatterning Assay: Quantitative Analysis of Protein–Protein Interactions
|
|---|---|
| Chapter number | 18 |
| Book title |
Proteomics
|
| Published in |
Methods in molecular biology, February 2017
|
| DOI | 10.1007/978-1-4939-6747-6_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6745-2, 978-1-4939-6747-6
|
| Authors |
Gerhard J. Schütz, Julian Weghuber, Peter Lanzerstorfer, Eva Sevcsik |
| Editors |
Lucio Comai, Jonathan E. Katz, Parag Mallick |
| Abstract |
Characterization, especially quantification, of protein interactions in live cells is usually not an easy endeavor. Here, we describe a straightforward method to identify and quantify the interaction of a membrane protein ("bait") and a fluorescently labeled interaction partner ("prey") (membrane-bound or cytosolic) in live cells using Total Internal Reflection Fluorescence microscopy. The bait protein is immobilized within patterns in the plasma membrane (e.g., via an antibody); the bait-prey interaction strength can be quantified by determining the prey bulk fluorescence intensity with respect to the bait patterns. This method is particularly suitable also for the analysis of weak, transient interactions that are not easily accessible with other methods. |
Mendeley readers
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|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Researcher | 3 | 20% |
| Student > Ph. D. Student | 2 | 13% |
| Unspecified | 2 | 13% |
| Student > Bachelor | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 3 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Unspecified | 2 | 13% |
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| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 4 | 27% |