Title |
Ubiquitin conjugation to Gag is essential for ESCRT-mediated HIV-1 budding
|
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Published in |
Retrovirology, July 2013
|
DOI | 10.1186/1742-4690-10-79 |
Pubmed ID | |
Authors |
Paola Sette, Kunio Nagashima, Robert C Piper, Fadila Bouamr |
Abstract |
HIV-1 relies on the host ESCRTs for release from cells. HIV-1 Gag engages ESCRTs by directly binding TSG101 or Alix. ESCRTs also sort ubiquitinated membrane proteins through endosomes to facilitate their lysosomal degradation. The ability of ESCRTs to recognize and process ubiquitinated proteins suggests that ESCRT-dependent viral release may also be controlled by ubiquitination. Although both Gag and ESCRTs undergo some level of ubiquitination, definitive demonstration that ubiquitin is required for viral release is lacking. Here we suppress ubiquitination at viral budding sites by fusing the catalytic domain of the Herpes Simplex UL36 deubiquitinating enzyme (DUb) onto TSG101, Alix, or Gag. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 1% |
Unknown | 80 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 30 | 37% |
Student > Bachelor | 10 | 12% |
Researcher | 8 | 10% |
Student > Master | 7 | 9% |
Student > Doctoral Student | 4 | 5% |
Other | 6 | 7% |
Unknown | 16 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 25 | 31% |
Agricultural and Biological Sciences | 19 | 23% |
Immunology and Microbiology | 9 | 11% |
Medicine and Dentistry | 3 | 4% |
Veterinary Science and Veterinary Medicine | 2 | 2% |
Other | 5 | 6% |
Unknown | 18 | 22% |