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RNA sequencing analysis of transcriptional change in the freshwater musselElliptio complanataafter environmentally relevant sodium chloride exposure

Overview of attention for article published in Environmental Toxicology & Chemistry, March 2017
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4 tweeters

Citations

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8 Dimensions

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18 Mendeley
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Title
RNA sequencing analysis of transcriptional change in the freshwater musselElliptio complanataafter environmentally relevant sodium chloride exposure
Published in
Environmental Toxicology & Chemistry, March 2017
DOI 10.1002/etc.3774
Pubmed ID
Authors

Laura S. Robertson, Heather S. Galbraith, Deborah Iwanowicz, Carrie J. Blakeslee, R. Scott Cornman

Abstract

To identify potential biomarkers of salt stress in a freshwater sentinel species, we examined transcriptional responses of the common mussel Elliptio complanata to controlled NaCl exposures. RNA-Seq of mantle tissue identified 481 transcripts differentially expressed in adult mussels exposed to 2 ppt NaCl (1.2 ppt chloride) for seven days, of which 290 had non-overlapping intervals. Differentially expressed gene categories included ion and transmembrane transport, oxidoreductase activity, maintenance of protein folding, and amino acid metabolism. The rate-limiting enzyme for synthesis of taurine, an amino acid frequently linked to osmotic stress in aquatic species, was up-regulated, as was the transmembrane ion pump sodium/potassium ATPase. These patterns confirm a primary transcriptional response to the experimental dose, albeit likely overlapping with non-specific secondary stress responses. Substantial involvement of the protein chaperone family Hsp70 and the water-transporting aquaporin family was not detected, however, in contrast to some studies in other bivalves. A subset of the most significantly regulated genes was confirmed by qPCR in an independent sample. Cluster analysis showed separation of mussels exposed to 2 ppt NaCl from control mussels in multivariate space, but mussels exposed to 1 ppt NaCl were largely indistinguishable from controls. Transcriptome-scale analysis of salt exposure under laboratory conditions efficiently identified candidate biomarkers for further functional analysis and field validation. This article is protected by copyright. All rights reserved.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 28%
Student > Ph. D. Student 3 17%
Student > Master 3 17%
Unspecified 2 11%
Professor 1 6%
Other 2 11%
Unknown 2 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 28%
Environmental Science 4 22%
Unspecified 2 11%
Biochemistry, Genetics and Molecular Biology 2 11%
Veterinary Science and Veterinary Medicine 1 6%
Other 0 0%
Unknown 4 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 September 2017.
All research outputs
#7,799,196
of 13,535,870 outputs
Outputs from Environmental Toxicology & Chemistry
#2,478
of 3,908 outputs
Outputs of similar age
#130,877
of 259,094 outputs
Outputs of similar age from Environmental Toxicology & Chemistry
#34
of 81 outputs
Altmetric has tracked 13,535,870 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,908 research outputs from this source. They receive a mean Attention Score of 4.5. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 259,094 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 81 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.