Title |
Sub-inhibitory concentrations of some antibiotics can drive diversification of Pseudomonas aeruginosa populations in artificial sputum medium
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Published in |
BMC Microbiology, July 2013
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DOI | 10.1186/1471-2180-13-170 |
Pubmed ID | |
Authors |
Elli A Wright, Joanne L Fothergill, Steve Paterson, Michael A Brockhurst, Craig Winstanley |
Abstract |
Pseudomonas aeruginosa populations within the cystic fibrosis lung exhibit extensive phenotypic and genetic diversification. The resultant population diversity is thought to be crucial to the persistence of infection and may underpin the progression of disease. However, because cystic fibrosis lungs represent ecologically complex and hostile environments, the selective forces driving this diversification in vivo remain unclear. We took an experimental evolution approach to test the hypothesis that sub-inhibitory antibiotics can drive diversification of P. aeruginosa populations. Replicate populations of P. aeruginosa LESB58 were cultured for seven days in artificial sputum medium with and without sub-inhibitory concentrations of various clinically relevant antibiotics. We then characterised diversification with respect to 13 phenotypic and genotypic characteristics. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 3 | 43% |
Unknown | 4 | 57% |
Demographic breakdown
Type | Count | As % |
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Scientists | 5 | 71% |
Members of the public | 2 | 29% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 4 | 3% |
France | 1 | <1% |
Colombia | 1 | <1% |
Spain | 1 | <1% |
Belgium | 1 | <1% |
Unknown | 130 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 27 | 20% |
Researcher | 26 | 19% |
Student > Master | 19 | 14% |
Student > Bachelor | 17 | 12% |
Student > Doctoral Student | 10 | 7% |
Other | 19 | 14% |
Unknown | 20 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 42 | 30% |
Medicine and Dentistry | 21 | 15% |
Immunology and Microbiology | 18 | 13% |
Biochemistry, Genetics and Molecular Biology | 12 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 4% |
Other | 16 | 12% |
Unknown | 24 | 17% |