Title |
Coupling of Mitochondrial Import and Export Translocases by Receptor-Mediated Supercomplex Formation
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Published in |
Cell, August 2013
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DOI | 10.1016/j.cell.2013.06.033 |
Pubmed ID | |
Authors |
Jian Qiu, Lena-Sophie Wenz, Ralf M. Zerbes, Silke Oeljeklaus, Maria Bohnert, David A. Stroud, Christophe Wirth, Lars Ellenrieder, Nicolas Thornton, Stephan Kutik, Sebastian Wiese, Agnes Schulze-Specking, Nicole Zufall, Agnieszka Chacinska, Bernard Guiard, Carola Hunte, Bettina Warscheid, Martin van der Laan, Nikolaus Pfanner, Nils Wiedemann, Thomas Becker |
Abstract |
The mitochondrial outer membrane harbors two protein translocases that are essential for cell viability: the translocase of the outer mitochondrial membrane (TOM) and the sorting and assembly machinery (SAM). The precursors of β-barrel proteins use both translocases-TOM for import to the intermembrane space and SAM for export into the outer membrane. It is unknown if the translocases cooperate and where the β-barrel of newly imported proteins is formed. We established a position-specific assay for monitoring β-barrel formation in vivo and in organello and demonstrated that the β-barrel was formed and membrane inserted while the precursor was bound to SAM. β-barrel formation was inhibited by SAM mutants and, unexpectedly, by mutants of the central import receptor, Tom22. We show that the cytosolic domain of Tom22 links TOM and SAM into a supercomplex, facilitating precursor transfer on the intermembrane space side. Our study reveals receptor-mediated coupling of import and export translocases as a means of precursor channeling. |
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Canada | 2 | 1% |
Czechia | 1 | <1% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 41 | 21% |
Student > Ph. D. Student | 36 | 19% |
Student > Bachelor | 23 | 12% |
Student > Master | 23 | 12% |
Student > Doctoral Student | 12 | 6% |
Other | 20 | 10% |
Unknown | 38 | 20% |
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---|---|---|
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Biochemistry, Genetics and Molecular Biology | 50 | 26% |
Medicine and Dentistry | 6 | 3% |
Chemistry | 6 | 3% |
Immunology and Microbiology | 5 | 3% |
Other | 11 | 6% |
Unknown | 40 | 21% |