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Single-cell transcriptome conservation in cryopreserved cells and tissues

Overview of attention for article published in Genome Biology (Online Edition), March 2017
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (95th percentile)

Mentioned by

news
2 news outlets
blogs
2 blogs
twitter
72 tweeters
facebook
2 Facebook pages

Citations

dimensions_citation
77 Dimensions

Readers on

mendeley
401 Mendeley
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Title
Single-cell transcriptome conservation in cryopreserved cells and tissues
Published in
Genome Biology (Online Edition), March 2017
DOI 10.1186/s13059-017-1171-9
Pubmed ID
Authors

Amy Guillaumet-Adkins, Gustavo Rodríguez-Esteban, Elisabetta Mereu, Maria Mendez-Lago, Diego A. Jaitin, Alberto Villanueva, August Vidal, Alex Martinez-Marti, Enriqueta Felip, Ana Vivancos, Hadas Keren-Shaul, Simon Heath, Marta Gut, Ido Amit, Ivo Gut, Holger Heyn

Abstract

A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.

Twitter Demographics

The data shown below were collected from the profiles of 72 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 401 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 <1%
Austria 1 <1%
Sweden 1 <1%
United Kingdom 1 <1%
Denmark 1 <1%
Japan 1 <1%
United States 1 <1%
Unknown 394 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 112 28%
Student > Ph. D. Student 102 25%
Student > Bachelor 31 8%
Student > Master 20 5%
Other 20 5%
Other 66 16%
Unknown 50 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 111 28%
Agricultural and Biological Sciences 100 25%
Medicine and Dentistry 57 14%
Immunology and Microbiology 24 6%
Neuroscience 14 3%
Other 32 8%
Unknown 63 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 66. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 January 2020.
All research outputs
#351,477
of 16,135,689 outputs
Outputs from Genome Biology (Online Edition)
#302
of 3,434 outputs
Outputs of similar age
#11,166
of 262,640 outputs
Outputs of similar age from Genome Biology (Online Edition)
#1
of 1 outputs
Altmetric has tracked 16,135,689 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,434 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.9. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,640 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them