Chapter title |
Analysis of TCR/CD3 Recycling at the Immune Synapse
|
---|---|
Chapter number | 10 |
Book title |
The Immune Synapse
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6881-7_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6879-4, 978-1-4939-6881-7
|
Authors |
Laura Patrussi, Cosima T. Baldari |
Editors |
Cosima T. Baldari, Michael L. Dustin |
Abstract |
Engagement of the T cell antigen receptor (TCR) by specific ligand bound to the major histocompatibility complex is the primary event that leads to the assembly of the immune synapse (IS). Central to this process is TCR clustering at the T cell-APC contact, which is achieved with the contribution of an endosomal pool that is delivered to the IS by polarized recycling. As the TCR recycling process has not been fully elucidated, we developed methods suitable to quantitate recycling to the plasma membrane of TCR/CD3 complexes that have been engaged at the cell surface and track their traffic through the intracellular vesicular compartments toward the IS. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Italy | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 18% |
Student > Bachelor | 2 | 18% |
Student > Master | 2 | 18% |
Other | 1 | 9% |
Student > Ph. D. Student | 1 | 9% |
Other | 0 | 0% |
Unknown | 3 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 4 | 36% |
Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Agricultural and Biological Sciences | 1 | 9% |
Unknown | 3 | 27% |