Chapter title |
Revealing the Role of Microscale Architecture in Immune Synapse Function Through Surface Micropatterning
|
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Chapter number | 17 |
Book title |
The Immune Synapse
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6881-7_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6879-4, 978-1-4939-6881-7
|
Authors |
Joung-Hyun Lee, Lance C. Kam |
Editors |
Cosima T. Baldari, Michael L. Dustin |
Abstract |
The immune synapse has emerged as a compelling example of structural complexity within cell-cell interfaces. This chapter focuses on the use of microcontact printing to isolate and investigate how spatial organization of signaling molecules drives the function of immune cells. In the process detailed here, multiple rounds of microcontact printing are combined to create patterned surfaces that control the relative spatial localization of CD3 and CD28 signaling in T cells, effectively replacing an antigen presenting cell with an engineered surface. A set of approaches used to address key issues of T cell activation are described and discussed. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
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Researcher | 1 | 100% |
Readers by discipline | Count | As % |
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Engineering | 1 | 100% |