Chapter title |
Spatial Control of Biological Ligands on Surfaces Applied to T Cell Activation
|
---|---|
Chapter number | 18 |
Book title |
The Immune Synapse
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6881-7_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6879-4, 978-1-4939-6881-7
|
Authors |
Haogang Cai, David Depoil, James Muller, Michael P. Sheetz, Michael L. Dustin, Shalom J. Wind, Cai, Haogang, Depoil, David, Muller, James, Sheetz, Michael P., Dustin, Michael L., Wind, Shalom J. |
Editors |
Cosima T. Baldari, Michael L. Dustin |
Abstract |
In this chapter, we present techniques, based on molecular-scale nanofabrication and selective self-assembly, for the presentation of biomolecules of interest (ligands, receptors, etc.) on a surface with precise spatial control and arbitrary geometry at the single-molecule level. Metallic nanodot arrays are created on glass coverslips and are then used as anchors for the immobilization of biological ligands via thiol linking chemistry. The nanodot size is controlled by both lithography and metallization. The reagent concentration in self-assembly can be adjusted to ensure single-molecule occupancy for a given dot size. The surrounding glass is backfilled by a protein-repellent layer to prevent nonspecific adsorption. Moreover, bifunctional surfaces are created, whereby a second ligand is presented on the background, which is frequently a requirement for simulating complex cellular functions involving more than one key ligand. This platform serves as a novel and powerful tool for molecular and cellular biology, e.g., to study the fundamental mechanisms of receptor-mediated signaling. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 2 | 20% |
Student > Doctoral Student | 1 | 10% |
Student > Ph. D. Student | 1 | 10% |
Researcher | 1 | 10% |
Professor > Associate Professor | 1 | 10% |
Other | 1 | 10% |
Unknown | 3 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Chemical Engineering | 1 | 10% |
Agricultural and Biological Sciences | 1 | 10% |
Earth and Planetary Sciences | 1 | 10% |
Chemistry | 1 | 10% |
Other | 1 | 10% |
Unknown | 3 | 30% |