Chapter title |
Probing Synaptic Biomechanics Using Micropillar Arrays
|
---|---|
Chapter number | 19 |
Book title |
The Immune Synapse
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6881-7_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6879-4, 978-1-4939-6881-7
|
Authors |
Weiyang Jin, Charles T. Black, Lance C. Kam, Morgan Huse |
Editors |
Cosima T. Baldari, Michael L. Dustin |
Abstract |
Recent insights into the importance of mechanosensing and force transmission at the immune synapse have spurred increased interest in the mechanical properties of leukocyte cell-cell interactions. In this chapter, we describe an imaging-based strategy for measuring cellular forces that utilizes optically transparent arrays of flexible micropillars. This approach has several distinct advantages over standard traction force microscopy, and we anticipate that it will prove very useful for investigators who wish not only to quantify ligand-induced forces with high spatiotemporal resolution but also to place those forces within the context of a broader cell biological response. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 40% |
Lecturer | 1 | 20% |
Unknown | 2 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 20% |
Immunology and Microbiology | 1 | 20% |
Engineering | 1 | 20% |
Unknown | 2 | 40% |