Chapter title |
Systems Imaging of the Immune Synapse
|
---|---|
Chapter number | 25 |
Book title |
The Immune Synapse
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6881-7_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6879-4, 978-1-4939-6881-7
|
Authors |
Rachel Ambler, Xiangtao Ruan, Robert F. Murphy, Christoph Wülfing |
Editors |
Cosima T. Baldari, Michael L. Dustin |
Abstract |
Three-dimensional live cell imaging of the interaction of T cells with antigen-presenting cells (APCs) visualizes the subcellular distributions of signaling intermediates during T cell activation at thousands of resolved positions within a cell. These information-rich maps of local protein concentrations are a valuable resource in understanding T cell signaling. Here, we describe a protocol for the efficient acquisition of such imaging data and their computational processing to create four-dimensional maps of local concentrations. This protocol allows quantitative analysis of T cell signaling as it occurs inside live cells with resolution in time and space across thousands of cells. |
Mendeley readers
Geographical breakdown
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 3 | 21% |
Researcher | 2 | 14% |
Student > Bachelor | 2 | 14% |
Professor | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 2 | 14% |
Chemical Engineering | 1 | 7% |
Other | 1 | 7% |
Unknown | 3 | 21% |